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Catalog Number | orb392215 |
---|---|
Category | Proteins |
Description | Recombinant human TrkB protein is produced by our Mammalian expression system and the target gene encoding Cys32-His430 is expressed with a 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.2. |
Purity | Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
Target | NTRK2 |
Entrez | 4915 |
UniProt ID | Q16620 |
Protein Sequence | CPTSCKCSAS RIWCSDPSPG IVAFPRLEPN SVDPENITEI FIANQKRLEI INEDDVEAYV GLRNLTIVDS GLKFVAHKAF LKNSNLQHIN FTRNKLTSLS RKHFRHLDLS ELILVGNPFT CSCDIMWIKT LQEAKSSPDT QDLYCLNESS KNIPLANLQI PNCGLPSANL AAPNLTVEEG KSITLSCSVA GDPVPNMYWD VGNLVSKHMN ETSHTQGSLR ITNISSDDSG KQISCVAENL VGEDQDSVNL TVHFAPTITF LESPTSDHHW CIPFTVKGNP KPALQWFYNG AILNESKYIC TKIHVTNHTE YHGCLQLDNP THMNNGDYTL IAKNEYGKDE KQISAHFMGW PGIDDGANPN YPDVIYEDYG TAANDIGDTT NRSNEIPSTD VTDKTGREHV DHHHHHH |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.2. |
Alternative names | TRKB; BDNF/NT-3 growth factors receptor; GP145-Trk Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 45.0 kDa after removal of the signal peptide. The apparent molecular mass of NTRK2-His is approximately 55-100 kDa due to glycosylation. | |
Mammalian |
Unconjugated | |
95% | |
45.2 kDa | |
Human TrkB, His Tag (orb257719) is expressed from human 293 cells (HEK293). It contains AA Cys 32 - His 430 (Accession # AAH31835). |
Unconjugated | |
98% | |
70.4 kDa | |
Human TrkB, Fc Tag (orb257720) is expressed from human 293 cells (HEK293). It contains AA Cys 32 - His 430 (Accession # AAH31835). |
IHC, IP, WB | |
Human | |
Rabbit | |
Monoclonal | |
Unconjugated |