Human Transferrin protein (Biotin)

SKU: orb346261

Description

Human Transferrin protein conjugated to Biotin

Images & Validation

• Tested Applications: DOT
• Application Notes:
  Human Transferrin Biotin has been tested in dot blot and is designed for Immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates.

Key Properties

• Source: Human
• Biological Origin: Human
• Isotype: Transferrin
• Purity: This product was prepared from normal serum by a multi-stage process that includes delipidation and selective precipitation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Human Transferrin and anti-Human Serum.
• Conjugation: Biotin

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 1.0 mg/ml
• Disclaimer: For research use only

Alternative Names

Human Transferrin Biotin Conjugation

Dot Blot of Biotin conjugated Human Transferrin. Antigen: Human Transferrin Biotin. Load: 3-fold serial dilution starting at 200 ng. Primary antibody: None. Secondary antibody: Peroxidase streptavidin secondary antibody at 1:40000 for 30 min at RT. Block: orb348637 for 60 min at RT.

Dot Blot results of Human Transferrin Biotin Conjugated. Antigen: Human Transferrin Biotin Conjugated. Blot loaded at 3 fold dilution: 1. 100 ng, 2. 33.3 ng, 3. 11.1 ng, 4. 3.70 ng, 5. 1.23 ng. Blocking: orb348637 Buffer for 30 minutes at RT. Secondary Antibody: Streptavidin-HRP (p/n orb348767) at 1:40000 for 30 min at RT.

Ex vivo fluorescence imaging of biodistribution of the nanoparticles in an orthotopic mouse model of PBT. Notes: (A) Fluorescent signal observed in the brains of four separate mice with PBT (1–24 hours) injected intravenously (via tail vein) with transferrin-coated Prussian blue nanoparticles (MnPB-ATxRd-Tf). A control mouse with PBT was not injected with nanoparticles. (B) Representative ex vivo fluorescence imaging of organ biodistribution of the nanoparticles at 3 hours post-injection. (C) Histograms quantifying the observed fluorescence biodistribution of the nanoparticles 3 hours post-injection. ROIs for intensity measurements are indicated by white dashed lines in (B). Abbreviations: PBT, pediatric brain tumor; MnPB, manganese-containing Prussian blue; ATxRd, Texas Red-labeled avidin; Tf, transferrin; ROI, region of interest; au, arbitrary units. Human Transferrin Biotin Conjugated (p/n orb346261).

Quick Database Links

UniProt

Q06AH7

RefSeq:AAB22049.1