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Catalog Number | orb428960 |
---|---|
Category | Proteins |
Description | Recombinant of human TPO (His) protein |
Form/Appearance | Sterile Filtered clear solution. |
Purity | Greater than 85% as determined by SDS-PAGE. |
Protein Sequence | SPAPPACDLR VLSKLLRDSH VLHSRLSQCP EVHPLPTPVL LPAVDFSLGE WKTQMEETKA QDILGAVTLL LEGVMAARGQ LGPTCLSSLL GQLSGQVRLL LGALQSLLGT QLPPQGRTTA HKDPNAIFLS FQHLLRGKVR FLMLVGGSTL CVRRAPPTTA VPSRTSLVLT LNELPNRTSGLLETNFTASA RTTGSGLLKW QQGFRAKIPG LLNQTSRSLD QIPGYLNRIH ELLNGTRGLF PGPSRRTLGA PDISSGTSDT GSLPPNLQPG YSPSPTHPPT GQYTLFPLPP TLPTPVVQLH PLLPDPSAPT PTPTSPLLNT SYTHSQNLSQ EGLEHHHHHH |
Source | Sf9, Insect cell |
Storage | Stability: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles |
Buffer/Preservatives | TPO protein solution (0.25mg/ml) contains phosphate buffered saline (pH7.4) and 10% glycerol. |
Alternative names | MPL Ligand, THCYT1, MPLLG, TPO, ML, Thrombopoietin Read more... |
Note | For research use only |
Application notes | Cytokines And Growth Factors |
Expiration Date | 6 months from date of receipt. |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 92.8 kDa after removal of the signal peptide. The apparent molecular mass of TPO-His is approximately 100-130 kDa due to glycosylation. | |
Mammalian |
> 90% as determined by SDS-PAGE | |
27.4 kDa |
> 99.9% | |
70-90 KDa (reducing condition) |
99.00% | |
30-40 kDa (reducing condition) |
Greater than 90.0% as determined by SDS-PAGE. | |
HEK293 cells |