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Human TOLLIP protein

SKU: orb392621

Description

Recombinant human TOLLIP protein is produced by our E. coli expression system and the target gene encoding Ala2-Pro274 is expressed with a 6His tag at the C-terminus.

Research Area

Cell Biology

Images & Validation

Tested ApplicationsELISA, MS, SDS-PAGE, WB
Application Notes
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

SourceE. coli
TargetTOLLIP
ReactivityHuman
EndotoxinsLess than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
PurityGreater than 95% as determined by reducing SDS-PAGE.
Protein SequenceATTVSTQRGP VYIGELPQDF LRITPTQQQR QVQLDAQAAQ QLQYGGAVGT VGRLNITVVQ AKLAKNYGMT RMDPYCRLRL GYAVYETPTA HNGAKNPRWN KVIHCTVPPG VDSFYLEIFD ERAFSMDDRI AWTHITIPES LRQGKVEDKW YSLSGRQGDD KEGMINLVMS YALLPAAMVM PPQPVVLMPT VYQQGVGYVP ITGMPAVCSP GMVPVALPPA AVNAQPRCSE EDLKAIQDMF PNMDQEVIRS VLEAQRGNKD AAINSLLQMG EEPVEHHHHH H

Storage & Handling

StorageLyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
Form/AppearanceLyophilized
Buffer/Preservatives0.2 μM filtered solution of 20mM PB, 150mM NaCl, 5mM EDTA, pH 7.2.
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Toll-interacting protein

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UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol

Human TOLLIP protein (orb392621)

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Available Sizes

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10 μg
$ 350.00
50 μg
$ 910.00
500 μg
$ 3,540.00
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