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Catalog Number | orb706860 |
---|---|
Category | Proteins |
Description | Recombinant Human CD256 Protein is produced by mammalian expression system and the target gene encoding Lys112-Leu250 is expressed with a Flag-His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | TNFSF13 |
Entrez | 8741 |
UniProt ID | O75888 |
Protein Sequence | DYKDDDDKHH HHHHGPGQVQ LQKKQHSVLH LVPINATSKD DSDVTEVMWQ PALRRGRGLQ AQGYGVRIQD AGVYLLYSQV LFQDVTFTMG QVVSREGQGR QETLFRCIRS MPSHPDRAYN SCYSAGVFHL HQGDILSVII PRARAKLNLS PHGTFLGFVK LGGGGSKKQH SVLHLVPINA TSKDDSDVTE VMWQPALRRG RGLQAQGYGV RIQDAGVYLL YSQVLFQDVT FTMGQVVSRE GQGRQETLFR CIRSMPSHPD RAYNSCYSAG VFHLHQGDIL SVIIPRARAK LNLSPHGTFL GFVKLGGGGS KKQHSVLHLV PINATSKDDS DVTEVMWQPA LRRGRGLQAQ GYGVRIQDAG VYLLYSQVLF QDVTFTMGQV VSREGQGRQE TLFRCIRSMP SHPDRAYNSC YSAGVFHLHQ GDILSVIIPR ARAKLNLSPH GTFLGFVKL |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
Alternative names | APRIL protein, TALL2 protein, ZTNF2 protein, Tumor Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
> 95% as determined by Tris-Bis PAGE | |
Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result. kDa |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 42.5 kDa after removal of the signal peptide. The apparent molecular mass of hFc-APRIL is approximately 35-55 kDa due to glycosylation. | |
Mammalian |
Human | |
0.78 ng/mL-50 ng/mL | |
0.195 ng/mL |