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Catalog Number | orb428921 |
---|---|
Category | Proteins |
Description | Recombinant of human TNFSF12 protein |
Form/Appearance | Filtered White lyophilized (freeze-dried) powder. |
Purity | Greater than 95% as determined by SDS-PAGE. |
Solubility (25°C) | It is recommended to reconstitute the lyophilized TNFSF12 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Protein Sequence | MHHHHHHRSA PKGRKTRARR AIAAHYEVHP RPGQDGAQAG VDGTVSGWEE ARINSSSPLR YNRQIGEFIV TRAGLYYLYC QVHFDEGKAV YLKLDLLVDG VLALRCLEEF SATAASSLGP QLRLCQVSGL LALRPGSSLR IRTLPWAHLK AAPFLTYFGL FQVH |
Source | Escherichia coli |
Biological Activity | The ED50, as measured in a proliferation assay using HUVECs, is less than 8ng/ml. |
Storage | Stability: Lyophilized TNFSF12 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TWEAK should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles |
Buffer/Preservatives | Lyophilized from a 0.2μm filtered solution in PBS. |
Alternative names | TWEAK, TNF-related weak inducer of apoptosis, TNFS Read more... |
Note | For research use only |
Application notes | Cytokines And Growth Factors |
Expiration Date | 6 months from date of receipt. |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 43.3 kDa after removal of the signal peptide. | |
Mammalian |
Greater than 90% as determined by SDS-PAGE. | |
38.9 kDa | |
E.coli |
Human | |
0.312 ng/ml-20 ng/ml | |
0.078 ng/ml |
98.00% | |
49.62 kDa (predicted). Due to enzyme lysis and glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result. |
98.00% | |
43.8 kDa (predicted); 34 and 47 kDa (reducing conditions) |