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Human TNFR2 Protein, His Tag
Catalog Number: orb257880
| Catalog Number | orb257880 |
|---|---|
| Category | Proteins |
| Description | TNF RI is also known as the p60 or p55 TNFR) and TNF RII (the p75 or p80 TNFR) are two distinct type I transmembrane glycoproteins that bind TNF with highaffinity.Both RI and RII are prototypic members of the TNF receptor superfamily and have been designated TNFRSF1A and TNFRSF1B, respectively. Human TNF RII cDNA encodes a 461 amino acid (aa) residue precursor protein with a 22 aa putative signal peptide, a 235 aa extracellular domain, a 20 aa transmembrane domain and a 174 aa cytoplasmic domain. TNFRII is expressed in fetal brain. The protein is produced naturally as a soluble form (sTNFRII). The soluble receptor inhibits TNFα action by competing with cell surface receptors in binding TNFα, thereby blocking its biologic effects. TNFRII is strongly expressed at the cartilage–pannus junction, and plays a major role in a subset of families with multiple cases of rheumatoid arthritis (RA). Further, high plasma levels of sTNFRII were significantly associated with increased incidence of coronary heart disease, independent of established cardiovascular risk factors, and seems to be useful for monitoring the inflammatory activity of sarcoidosis. |
| Target | TNFR2 |
| Conjugation | Unconjugated |
| Tag | C-6×His |
| Reactivity | Human |
| Form/Appearance | Powder |
| Buffer/Preservatives | PBS, pH 7.4 |
| Purity | 95% |
| Protein Sequence | AAA36755 |
| UniProt ID | P20333 |
| MW | 26.0 kDa |
| Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 26.0 kDa. The protein migrates as 35-45 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Expression System | HEK293 |
| Endotoxins | 1.0 EU per μg |
| Biological Origin | Human |
| Expression Region | Leu 23 - Asp 257 |
| Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
| Research Area | Disease Biomarkers |
| Note | For research use only |
| NCBI | AAA36755 |
| Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human TNFR2 protein
- Item 1 of 1
Human TNFRSF1B Protein, His Tag [orb1743294]
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 26.0 kDa after removal of the signal peptide. The apparent molecular mass of TNFRSF1B-His is approximately 35-55 kDa due to glycosylation.
E.coli
50 μg, 10 μg, 100 μg 
Recombinant Human TNFR2 Protein, mFc His Tag [orb1550589]
> 95% by Tris-Bis PAGE, > 95% by SEC-HPLC
Recombinant Human TNFR2 Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Leu23-Asp257) of Human TNFR2 fused with mFc at the C-terminal.
100 μgRecombinant Human TNFRSF1B Protein, His Tag [orb1551506]
≥90% as determined by SDS-PAGE
This protein contains the human TNFRSF1B(Pro24-Thr206) was fused with the C-terminal His Tag and expressed in E. coli.
100 μg, 50 μgRecombinant Human TNF RII/TNFRSF1B Protein, His Tag [orb1551524]
≥90% as determined by SDS-PAGE
This protein contains the human TNFRSF1B(Pro24-Thr206) was fused with the C-terminal His Tag and expressed in E. coli.
100 μg, 50 μgRecombinant Human TNFR2 Protein, His Tag [orb1550925]
> 95% by SDS-PAGE.
Recombinant Human TNFR2 Protein is produced by HEK293 Cells expression system. The target protein is expressed with sequence (Leu 23 - Asp 257) of human TNFR2 (Accession #NP_001057.1) fused with a 6��His Tag at the C-terminus.
50 μg, 100 μg
