Human TNF protein

• Catalog Number: orb392577
• Category: Proteins
• Description: Recombinant human TNF protein is produced by our E. coli using rDNA technology. The protein consists of three identical polypeptide chains of 158 amino acids combined to form a compact bell-shaped homotrimer. The individual subunits have a relative molecular mass of 17484 Daltons each. TNF-alpha is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells.
• Tested applications: ELISA, MS, SDS-PAGE, WB
• Reactivity: Human
• Form/Appearance: Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.0.
• Purity: Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
• Target: TNF
• Entrez: 7124
• UniProt ID: P01375
• Protein Sequence: MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA EAKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL
• Source: E. coli
• Endotoxins: Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
• Storage: Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
• Buffer/Preservatives: Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.0.
• Alternative names: TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TN
• Note: For research use only
• Application notes: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.