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Catalog Number | orb1733281 |
---|---|
Category | Proteins |
Description | Human TNF-beta Protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Leu 35 - Leu 205 (Accession # P01374). |
Target | TNF-beta |
Conjugation | Unconjugated |
Tag | C-10×His |
Reactivity | Human |
Form/Appearance | Powder |
Buffer/Preservatives | PBS, pH 7.4 |
Purity | 90% |
UniProt ID | P01374 |
MW | 20.5 kDa |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 20.5 kDa. The protein migrates as 26-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expression System | HEK293 |
Endotoxins | 1.0 EU per μg |
Biological Origin | Human |
Biological Activity | Immobilized Human TNFR1, Fc Tag (Cat. No. TN1-H5251) at 5 μg/mL (100 μL/well) can bind Human TNF-beta Protein, His Tag (Cat. No. ) with a linear range of 1-20 ng/mL (QC tested). |
Expression Region | Leu 35 - Leu 205 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Note | For research use only |
Immobilized Human TNFR1, Fc Tag (Cat. No. orb257878) at 5 μg/mL (100 μL/well) can bind Human TNF-beta Protein, His Tag (Cat. No. orb1733281) with a linear range of 1-20 ng/mL (QC tested).
Greater than 90% as determined by SDS-PAGE. | |
Escherichia Coli |