You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb80621 |
---|---|
Category | Proteins |
Description | Recombinant of Human TNF b protein (His Tag) |
Form/Appearance | Sterile Filtered clear solution. |
Buffer/Preservatives | TNF-b protein solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 30% glycerol, 0.1M NaCl and 1mM DTT. |
Purity | Greater than 90% as determined by SDS-PAGE. |
Protein Sequence | MGSSHHHHHH SSGLVPRGSH MLPGVGLTPS AAQTARQHPK MHLAHSTLKP AAHLIGDPSK QNSLLWRANT DRAFLQDGFS LSNNSLLVPT SGIYFVYSQV VFSGKAYSPK ATSSPLYLAH EVQLFSSQYP FHVPLLSSQK MVYPGLQEPW LHSMYHGAAF QLTQGDQLST HTDGIPHLVL SPSTVFFGAF AL |
Application notes | Cytokines And Growth Factors |
Source | Escherichia Coli |
Biological Activity | Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is < 0.3 ng/ml. |
Storage | Stability: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles |
Alternative names | Lymphotoxin-alpha, LT-alpha, TNF-beta, Tumor necro Read more... |
Note | For research use only |
> 95% as determined by Bis-Tris PAGE | |
8.9 kDa (predicted). Due to glycosylation, the protein migrates to 12 kDa and 15-17 kDa based on Tris-Bis PAGE result. |
98.00% | |
29.5 kDa (predicted). Due to glycosylation, the protein migrates to 35-50 kDa based on Tris-Bis PAGE result. |
98.00% | |
29.5 kDa (predicted). Due to glycosylation, the protein migrates to 30-48 kDa based on Tris-Bis PAGE result. |
> 95% | |
52.4 kDa (predicted). Due to glycosylation, the protein migrates to 53-60 kDa based on Tris-Bis PAGE result. |
98.00% | |
54.2 kDa (predicted). Due to glycosylation, the protein migrates to 55-68 kDa based on Tris-Bis PAGE result. |