Human TNF a Protein (His Tag)

• Catalog Number: orb169442
• Category: Proteins
• Description: Recombinant of Human TNF a protein (His Tag)
• Form/Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Buffer/Preservatives: Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.0.
• Purity: Greater than 97.0% as determined by SDS-PAGE and HPLC analyses.
• Protein Sequence: MHHHHHHVRS SSRTPSDKPV AHVVANPQAE GQLQWLNRRA NALLANGVEL RDNQLVVPSE GLYLIYSQVL FKGQGCPSTH VLLTHTISRI AVSYQTKVNL LSAIKSPCQR ETPEGAEAKP WYEPIYLGGV FQLEKGDRLS AEINRPDYLD FAESGQVYFG IIAL
• Application notes: Cytokines And Growth Factors
• Source: Escherichia Coli
• Biological Activity: The ED50 was determined in the presence of actinomycin D by a cytotoxicity assay using murine L929 cells is 2.0 × 107IU/mg.
• Solubility (25°C): It is recommended to reconstitute the lyophilized TNF-α in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
• Storage: Stability: Lyophilized TNF-α although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNF-α should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles
• Alternative names: TNF-alpha, Tumor necrosis factor ligand superfamil
• Note: For research use only