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Catalog Number | orb428857 |
---|---|
Category | Proteins |
Description | Recombinant of human TIMP2 (HEK) protein |
Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Purity | Greater than 95.0% as determined by SDS-PAGE. |
Solubility (25°C) | It is recommended to reconstitute the lyophilized TIMP2 in sterile assay buffer (50mM Tris, 10mM CaCl2, 150mM NaCl, 0.05% Brij-35, PH 7.5) not less than 100µg/ml. |
Source | HEK293 Cells |
Biological Activity | The IC50 of 3 nM is measured by its ability to inhibit recombinant human MMP-2 cleavage of the colorimetric peptide substrate, Mca-PLGL-DpaAR-NH2. |
Storage | Stability: Lyophilized TIMP2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TIMP2 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles |
Buffer/Preservatives | The TIMP2 protein was lyophilized after extensive dialysis against PBS. |
Alternative names | TIMP metallopeptidase inhibitor 2, CSC-21K, tissue Read more... |
Note | For research use only |
Application notes | Enzymes |
Expiration Date | 6 months from date of receipt. |