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Catalog Number | orb392565 |
---|---|
Category | Proteins |
Description | Recombinant human TIE1 protein is produced by our Mammalian expression system and the target gene encoding Ala22-Gln760 is expressed with a 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM MES, 150mM NaCl, pH 5.5 |
Purity | Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
Target | TIE1 |
Entrez | 7075 |
UniProt ID | P35590 |
Protein Sequence | AVDLTLLANL RLTDPQRFFL TCVSGEAGAG RGSDAWGPPL LLEKDDRIVR TPPGPPLRLA RNGSHQVTLR GFSKPSDLVG VFSCVGGAGA RRTRVIYVHN SPGAHLLPDK VTHTVNKGDT AVLSARVHKE KQTDVIWKSN GSYFYTLDWH EAQDGRFLLQ LPNVQPPSSG IYSATYLEAS PLGSAFFRLI VRGCGAGRWG PGCTKECPGC LHGGVCHDHD GECVCPPGFT GTRCEQACRE GRFGQSCQEQ CPGISGCRGL TFCLPDPYGC SCGSGWRGSQ CQEACAPGHF GADCRLQCQC QNGGTCDRFS GCVCPSGWHG VHCEKSDRIP QILNMASELE FNLETMPRIN CAAAGNPFPV RGSIELRKPD GTVLLSTKAI VEPEKTTAEF EVPRLVLADS GFWECRVSTS GGQDSRRFKV NVKVPPVPLA APRLLTKQSR QLVVSPLVSF SGDGPISTVR LHYRPQDSTM DWSTIVVDPS ENVTLMNLRP KTGYSVRVQL SRPGEGGEGA WGPPTLMTTD CPEPLLQPWL EGWHVEGTDR LRVSWSLPLV PGPLVGDGFL LRLWDGTRGQ ERRENVSSPQ ARTALLTGLT PGTHYQLDVQ LYHCTLLGPA SPPAHVLLPP SGPPAPRHLH AQALSDSEIQ LTWKHPEALP GPISKYVVEV QVAGGAGDPL WIDVDRPEET STIIRGLNAS TRYLFRMRAS IQGLGDWSNT VEESTLGNGL QAEGPVQESR AAEEGLDQQV DHHHHHH |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM MES, 150mM NaCl, pH 5.5 |
Alternative names | TIE; Tyrosine-protein kinase receptor Tie-1 Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Human | |
0.94 ng/mL-60 ng/mL | |
0.23 ng/mL |
Human | |
0.16-10ng/mL | |
0.1 ng/mL |
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