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Catalog Number | orb428811 |
---|---|
Category | Tools |
Description | Recombinant of human TGFB3 protein |
Form/Appearance | Sterile Filtered clear solution. |
Purity | Greater than 95.0% as determined by SDS-PAGE. |
Protein Sequence | MALDTNYCFRN LEENCCVRPL YIDFRQDLGW KWVHEPKGYY ANFCSGPCPY LRSADTTHST VLGLYNTLNP EASASPCCVP QDLEPLTILY YVGRTPKVEQ LSNMVVKSCK CS |
Source | Escherichia Coli |
Biological Activity | The activity is determined by the ability to induce chondrogenic differentiation. |
Storage | Stability: TGF-beta 3 although stable at room temperature for 1 week, should be stored at 4°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) |
Buffer/Preservatives | The protein solution contains 20% Ethanol and 10mM Acetic acid (AcOH). |
Alternative names | Transforming Growth Factor-beta3, TGFB3, ARVD, FLJ Read more... |
Note | For research use only |
Application notes | Protein content: Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 1.718 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of TGF-b 3 as a Reference Standard |
Expiration Date | 12 months from date of receipt. |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
Human cells |
Greater than 90% as determined by SDS-PAGE. | |
48.1 kDa | |
E.coli |
Greater than 95% as determined by SDS-PAGE. | |
12.7 kDa | |
Mammalian cell |
Greater than 90% as determined by SDS-PAGE. | |
16.7 kDa | |
E.coli |
> 95% by SDS-PAGE. | |
KMP1652, Recombinant Human TGF-beta 3/TGFB3 Protein is produced by Human Cells expression system. The target protein is expressed with sequence (Ala301-Ser412(Tyr340Phe)) of human TGF-beta 3/TGFB3 (Accession #P10600). |
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