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Catalog Number | orb392532 |
---|---|
Category | Proteins |
Description | Recombinant human SULT1B1 protein is produced by our E. coli expression system and the target gene encoding Met1-Ile296 is expressed with a 6His tag at the N-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Liquid in a 0.2 μM filtered solution of 20mM Tris HCl, 100mM Nacl, pH 8.0. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | SULT1B1 |
Entrez | 27284 |
UniProt ID | O43704 |
Protein Sequence | MNHKVHHHHH HMLSPKDILR KDLKLVHGYP MTCAFASNWE KIEQFHSRPD DIVIATYPKS GTTWVSEIID MILNDGDIEK CKRGFITEKV PMLEMTLPGL RTSGIEQLEK NPSPRIVKTH LPTDLLPKSF WENNCKMIYL ARNAKDVSVS YYHFDLMNNL QPFPGTWEEY LEKFLTGKVA YGSWFTHVKN WWKRKEEHPI LFLYYEDMKE NPKEEIKKII RFLEKNLNDE ILDRIIHHTS FEVMKDNPLV NYTHLPTTVM DHSKSPFMRK GTAGDWKNYF TVAQNEKFDA IYETEMSKTA LQFRTEI |
Source | E. coli |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | Liquid in a 0.2 μM filtered solution of 20mM Tris HCl, 100mM Nacl, pH 8.0. |
Alternative names | ST1B2; SULT1B2; Sulfotransferase family cytosolic Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Greater than 95% as determined by SDS-PAGE. | |
E.coli |
98.00% | |
35.7 kDa (predicted); 34 kDa (reducing conditions) |
98.00% | |
42 kDa (predicted); 44 kDa (reducing conditions) |
≥90% as determined by SDS-PAGE | |
This protein contains the human SULT1B1(Met1-Ile296) was fused with the N-terminal His Tag and expressed in E. coli. |