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Catalog Number | orb80102 |
---|---|
Category | Proteins |
Description | Human Stem Cell Factor protein |
Tested applications | FA, HPLC, SDS-PAGE |
Purity | > 98.0% as determined by RP-HPLC and analysis by SDS-PAGE |
Conjugation | Unconjugated |
Target | Human Stem Cell Factor |
Solubility (25°C) | It is recommended to reconstitute the lyophilized Stem Cell Factor in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
Protein Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Met-Glu-Gly-Ile-Cys. |
Biological Origin | E.coli |
Biological Activity | The ED50 as determined by the dose-dependant stimulation of Human TF-1 cells is 2 ng/ml, corresponding to Specific Activity of 500,000 IU/mg. |
Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
Buffer/Preservatives | Lyophilized from concentrated (1mg/ml) solution in water containing 10mM Acetic acid. |
Alternative names | Kit ligand Precursor protein, C-kit ligand protein Read more... |
Note | For research use only |
Application notes | Experiment Notes: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.52 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using standard solution of Stem Cell Factor as Reference Standard. |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human Stem Cell Factor protein
Unconjugated | |
95% | |
18.5 kDa | |
Human SCF (26-189), premium grade (orb1496171) is expressed from human 293 cells (HEK293). It contains AA Glu 26 - Ala 189 (Accession # P21583-1). |
Unconjugated | |
90% | |
20.3 kDa | |
Human SCF, His Tag (orb1743048) is expressed from human 293 cells (HEK293). It contains AA Glu 26 - Ala 189 (Accession # P21583-1). |
> 97% as determined by SDS-PAGE and HPLC. | |
18.5 kDa | |
E.Coli |
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