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Catalog Number | orb392510 |
---|---|
Category | Proteins |
Description | Recombinant human SPESP1 protein is produced by our mammalian expression system and the target gene encoding Tyr20-Tyr350 is expressed with a 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | SPESP1 |
Entrez | 246777 |
UniProt ID | Q6UW49 |
Protein Sequence | YPSITVTPDE EQNLNHYIQV LENLVRSVPS GEPGREKKSN SPKHVYSIAS KGSKFKELVT HGDASTENDV LTNPISEETT TFPTGGFTPE IGKKKHTEST PFWSIKPNNV SIVLHAEEPY IENEEPEPEP EPAAKQTEAP RMLPVVTESS TSPYVTSYKS PVTTLDKSTG IGISTESEDV PQLSGETAIE KPEEFGKHPE SWNNDDILKK ILDINSQVQQ ALLSDTSNPA YREDIEASKD HLKRSLALAA AAEHKLKTMY KSQLLPVGRT SNKIDDIETV INMLCNSRSK LYEYLDIKCV PPEMREKAAT VFNTLKNMCR SRRVTALLKV YVDHHHHHH |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
Alternative names | Sperm equatorial segment protein 1; ESP; Equatoria Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
≥90% as determined by SDS-PAGE | |
This protein contains the human SPESP1(Tyr20-Tyr350) was fused with the C-terminal His Tag and expressed in Mammalian cells. |
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