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Catalog Number | orb392508 |
---|---|
Category | Proteins |
Description | Recombinant human SOST protein is produced by our mammalian expression system and the target gene encoding Gln24-Tyr213 is expressed with a 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM Tris, 250mM NaCl, pH 8.0. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | SOST |
Entrez | 50964 |
UniProt ID | Q9BQB4 |
Protein Sequence | QGWQAFKNDA TEIIPELGEY PEPPPELENN KTMNRAENGG RPPHHPFETK DVSEYSCREL HFTRYVTDGP CRSAKPVTEL VCSGQCGPAR LLPNAIGRGK WWRPSGPDFR CIPDRYRAQR VQLLCPGGEA PRARKVRLVA SCKCKRLTRF HNQSELKDFG TEAARPQKGR KPRPRARSAK ANQAELENAY HHHHHH |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM Tris, 250mM NaCl, pH 8.0. |
Alternative names | Sclerostin Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
90% | |
22.3 kDa | |
Human SOST, His Tag (orb257550) is expressed from human 293 cells (HEK293). It contains AA Gln 24 - Tyr 213 (Accession # AAI01087.1). |
ELISA, WB | |
Greater than 95% as determined by SDS-PAGE | |
20.8 kDa | |
E.Coli |
99.60% | |
22.5 kDa (predicted); 29.9 kDa (reducing condition, due to glycosylation) |
98.00% | |
22.5 kDa (predicted) |
98.00% | |
24.2 kDa (predicted). Due to glycosylation,the protein migrates to 28-40 kDa based on Tris-Bis PAGE result. |