Human Sonic HedgeHog protein

• Catalog Number: orb80100
• Category: Proteins
• Description: Sonic HedgeHog Recombinant Human produced in E.coli is a single, non-glycosylated polypeptide chain containing 175 amino acids and having a molecular mass of 19,683 Dalton. The Human Sonic Hedgehog is 99% homologous to the mouse gene.Cysteine at position 25 has been substituted with Ile.The Sonic HedgeHog is purified by proprietary chromatographic techniques.
• Target: SHH
• Conjugation: Unconjugated
• Buffer/Preservatives: Lyophilized from concentrated (1mg/ml) solution in water containing 10mM sodium Phosphate buffer pH-7.5.
• Purity: > 97.0% as determined by RP-HPLC and analysis by SDS-PAGE
• Protein Sequence: IIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKITRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG.
• Tested applications: FA, HPLC, SDS-PAGE
• Biological Origin: E.coli
• Biological Activity: The ED50, determined by the dose-dependent induction of alkaline phosphatase production by C3H10T1/2 fibroblasts is 760ng/ml.
• Solubility (25°C): It is recommended to reconstitute the lyophilized Sonic HedgeHog with sterile water at concentration of 0.1 0.5 mg/ml, which can be further diluted into other aqueous solutions.
• Storage: Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
• Alternative names: SHH protein, HHG-1 protein, HHG1 protein, Sonic he
• Note: For research use only

SDS-PAGE analysis of Human Sonic HedgeHog protein