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Human SIRT1 protein

SKU: orb358855

Description

This Human SIRT1 protein spans the amino acid sequence from region 2-747aa. Purity: Greater than 90% as determined by SDS-PAGE.

Research Area

Epigenetics & Chromatin

Customer Validated Data

The data below is submitted by researchers worldwide. We provide this transparency to help you decide if this product works for your specific application or species, even if we haven't tested it ourselves.
Success by Application
Reactivity Distribution

Latest Experiments

2 Results
Dilution
Sample
Summary
In vitro
Human
1 μmol/L
Cell lysates and cell culture media
Human SIRT1 protein used as a...
In vitro
Human
10 μM
In vitro reaction mixtures
Human SIRT1 protein used as a...

Images & Validation

Application Notes
This is His-tag protein

Key Properties

SourceE.coli
Biological OriginHomo sapiens (Human)
TagN-terminal 6xHis-tagged
Molecular Weight85.5 kDa
Expression Region2-747aa
Protein LengthFull Length of Mature Protein
Protein SequenceADEAALALQPGGSPSAAGADREAASSPAGEPLRKRPRRDGPGLERSPGEPGGAAPEREVPAAARGCPGAAAAALWREAEAEAAAAGGEQEAQATAAAGEGDNGPGLQGPSREPPLADNLYDEDDDDEGEEEEEAAAAAIGYRDNLLFGDEIITNGFHSCESDEEDRASHASSSDWTPRPRIGPYTFVQQHLMIGTDPRTILKDLLPETIPPPELDDMTLWQIVINILSEPPKRKKRKDINTIEDAVKLLQECKKIIVLTGAGVSVSCGIPDFRSRDGIYARLAVDFPDLPDPQAMFDIEYFRKDPRPFFKFAKEIYPGQFQPSLCHKFIALSDKEGKLLRNYTQNIDTLEQVAGIQRIIQCHGSFATASCLICKYKVDCEAVRGDIFNQVVPRCPRCPADEPLAIMKPEIVFFGENLPEQFHRAMKYDKDEVDLLIVIGSSLKVRPVALIPSSIPHEVPQILINREPLPHLHFDVELLGDCDVIINELCHRLGGEYAKLCCNPVKLSEITEKPPRTQKELAYLSELPPTPLHVSEDSSSPERTSPPDSSVIVTLLDQAAKSNDDLDVSESKGCMEEKPQEVQTSRNVESIAEQMENPDLKNVGSSTGEKNERTSVAGTVRKCWPNRVAKEQISRRLDGNQYLFLPPNRYIFHGAEVYSDSEDDVLSSSSCGSNSDSGTCQSPSLEEPMEDESEIEEFYNGLEDEPDVPERAGGAGFGTDGDDQEAINEAISVKQEVTDMNYPSNKS
PurityGreater than 90% as determined by SDS-PAGE.

Storage & Handling

StorageThe shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
Form/AppearanceLiquid or Lyophilized powder
Buffer/PreservativesIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Regulatory protein SIR2 homolog 1 SIR2-like protein 1 Short name, hSIR2 Cleaved into the following chain, SirtT1 75KDA fragment Short name, 75SirT1

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Human SIRT1 protein

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

UniProt Details

No UniProt data available

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Protocol Information

Human SIRT1 protein (orb358855)

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20 μg
$ 250.00
100 μg
$ 440.00
1 mg
$ 1,840.00
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