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Catalog Number | orb392486 |
---|---|
Category | Proteins |
Description | Recombinant human SIRPA protein is produced by our Mammalian expression system and the target gene encoding Glu31-Arg370 is expressed with a 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.2. |
Purity | Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
Target | SIRPA |
Entrez | 140885 |
UniProt ID | P78324 |
Protein Sequence | EEELQVIQPD KSVSVAAGES AILHCTVTSL IPVGPIQWFR GAGPARELIY NQKEGHFPRV TTVSESTKRE NMDFSISISN ITPADAGTYY CVKFRKGSPD TEFKSGAGTE LSVRAKPSAP VVSGPAARAT PQHTVSFTCE SHGFSPRDIT LKWFKNGNEL SDFQTNVDPV GESVSYSIHS TAKVVLTRED VHSQVICEVA HVTLQGDPLR GTANLSETIR VPPTLEVTQQ PVRAENQVNV TCQVRKFYPQ RLQLTWLENG NVSRTETAST VTENKDGTYN WMSWLLVNVS AHRDDVKLTC QVEHDGQPAV SKSHDLKVSA HPKEQGSNTA AENTGSNERN VDHHHHHH |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.2. |
Alternative names | BIT; MFR; MYD1; PTPNS1; SHPS1; SIRP; Tyrosine-prot Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
95% | |
38.5 kDa | |
Human SIRP alpha, His Tag (orb257842) is expressed from human 293 cells (HEK293). It contains AA Glu 31 - Arg 370 (Accession # NP_001035111). |
Unconjugated | |
95% | |
63.9 kDa | |
Human SIRP alpha, Fc Tag (orb257843) is expressed from human 293 cells (HEK293). It contains AA Glu 31 - Arg 370 (Accession # NP_001035111). |
Unconjugated | |
95% | |
63.0 kDa | |
Human SIRP alpha, Mouse IgG1 Fc Tag (orb257844) is expressed from human 293 cells (HEK293). It contains AA Glu 31 - Arg 370 (Accession # NP_001035111). |
Unconjugated | |
95% | |
15.6 kDa | |
Human CD47, His Tag (orb257293) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Pro 139 (Accession # NP_942088). |
Unconjugated | |
95% | |
40.4 kDa | |
Human CD47, Fc Tag (orb257295) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Pro 139 (Accession # NP_942088). |
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