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Catalog Number | orb392484 |
---|---|
Category | Proteins |
Description | Recombinant human SIGLEC9 protein is produced by our Mammalian expression system and the target gene encoding Gln18-Gly348 is expressed with a Fc tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of PBS, pH 7.4. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | SIGLEC9 |
Entrez | 27180 |
UniProt ID | Q9Y336 |
Protein Sequence | QTSKLLTMQS SVTVQEGLCV HVPCSFSYPS HGWIYPGPVV HGYWFREGAN TDQDAPVATN NPARAVWEET RDRFHLLGDP HTENCTLSIR DARRSDAGRY FFRMEKGSIK WNYKHHRLSV NVTALTHRPN ILIPGTLESG CPQNLTCSVP WACEQGTPPM ISWIGTSVSP LDPSTTRSSV LTLIPQPQDH GTSLTCQVTF PGASVTTNKT VHLNVSYPPQ NLTMTVFQGD GTVSTVLGNG SSLSLPEGQS LRLVCAVDAV DSNPPARLSL SWRGLTLCPS QPSNPGVLEL PWVHLRDEAE FTCRAQNPLG SQQVYLNVSL QSKATSGVTQ GVDDIEGRMD EPKSCDKTHT CPPCPAPELL GGPSVFLFPP KPKDTLMISR TPEVTCVVVD VSHEDPEVKF NWYVDGVEVH NAKTKPREEQ YNSTYRVVSV LTVLHQDWLN GKEYKCKVSN KALPAPIEKT ISKAKGQPRE PQVYTLPPSR EEMTKNQVSL TCLVKGFYPS DIAVEWESNG QPENNYKTTP PVLDSDGSFF LYSKLTVDKS RWQQGNVFSC SVMHEALHNH YTQKSLSLSP GK |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of PBS, pH 7.4. |
Alternative names | Sialic acid-binding Ig-like lectin 9; Siglec-9; CD Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Greater than 95% as determined by SDS-PAGE. | |
37.5 kDa | |
Mammalian cell |
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC | |
Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result. |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 36.9 kDa after removal of the signal peptide. The apparent molecular mass of SIGLEC9-His is approximately 55-70 kDa due to glycosylation. | |
Mammalian |
> 95% as determined by Tris-Bis PAGE | |
Due to glycosylation, the protein migrates to 80-110 kDa based on Tris-Bis PAGE result. |