Human SIAE protein

SKU: orb392483

Description

Recombinant human SIAE protein is produced by our mammalian expression system and the target gene encoding Ile24-Lys523 is expressed with a 6His tag at the C-terminus.

Images & Validation

• Tested Applications: ELISA, MS, SDS-PAGE, WB
• Application Notes:
  Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

• Source: Human cells
• Reactivity: Human
• Protein Sequence: IGFRFASYIN NDMVLQKEPA GAVIWGFGTP GATVTVTLRQ GQETIMKKVT SVKAHSDTWM VVLDPMKPGG PFEVMAQQTL EKINFTLRVH DVLFGDVWLC SGQSNMQMTV LQIFNATREL SNTAAYQSVR ILSVSPIQAE QELEDLVAVD LQWSKPTSEN LGHGYFKYMS AVCWLFGRHL YDTLQYPIGL IASSWGGTPI EAWSSGRSLK ACGVPKQGSI PYDSVTGPSK HSVLWNAMIH PLCNMTLKGV VWYQGESNIN YNTDLYNCTF PALIEDWRET FHRGSQGQTE RFFPFGLVQL SSDLSKKSSD DGFPQIRWHQ TADFGYVPNP KMPNTFMAVA MDLCDRDSPF GSIHPRDKQT VAYRLHLGAR ALAYGEKNLT FEGPLPEKIE LLAHKGLLNL TYYQQIQVQK KDNKIFEISC CSDHRCKWLP ASMNTVSTQS LTLAIDSCHG TVVALRYAWT TWPCEYKQCP LYHPSSALPA PPFIAFITDQ GPGHQSNVAK VDHHHHHH
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxins: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Storage & Handling

• Storage: Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Form/Appearance: Liquid in a 0.2 μM filtered solution of 20mM Tris-HCl, 150mM NaCl, 10% Glycerol, pH 7.5.
• Disclaimer: For research use only

Alternative Names

YSG2; Sialate O-acetylesterase; H-Lse; Sialic acid-specific 9-O-acetylesterase

Quick Database Links

UniProt

Q9HAT2

NCBI Gene ID

54414