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Catalog Number | orb392481 |
---|---|
Category | Proteins |
Description | Recombinant human SHMT1 protein is produced by our mammalian expression system and the target gene encoding Met3-Phe483 is expressed with a 6His tag at the C-terminus. |
Target | SHMT1 |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Protein Sequence | MPVNGAHKDA DLWSSHDKML AQPLKDSDVE VYNIIKKESN RQRVGLELIA SENFASRAVL EALGSCLNNK YSEGYPGQRY YGGTEFIDEL ETLCQKRALQ AYKLDPQCWG VNVQPYSGSP ANFAVYTALV EPHGRIMGLD LPDGGHLTHG FMTDKKKISA TSIFFESMPY KVNPDTGYIN YDQLEENARL FHPKLIIAGT SCYSRNLEYA RLRKIADENG AYLMADMAHI SGLVAAGVVP SPFEHCHVVT TTTHKTLRGC RAGMIFYRKG VKSVDPKTGK EILYNLESLI NSAVFPGLQG GPHNHAIAGV AVALKQAMTL EFKVYQHQVV ANCRALSEAL TELGYKIVTG GSDNHLILVD LRSKGTDGGR AEKVLEACSI ACNKNTCPGD RSALRPSGLR LGTPALTSRG LLEKDFQKVA HFIHRGIELT LQIQSDTGVR ATLKEFKERL AGDKYQAAVQ ALREEVESFA SFFPLPGLPD FVDHHHHHH |
UniProt ID | P34896 |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Source | Human cells |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Alternative names | Serine hydroxymethyltransferase cytosolic; SHMT; G Read more... |
Note | For research use only |
Entrez | 6470 |
FC, ICC, IF, IHC, WB | |
Human, Monkey, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
> 90% as determined by SDS-PAGE. | |
62.89 kDa |
ELISA, WB | |
Greater than 95% as determined by SDS-PAGE | |
53 kDa | |
E.Coli |
FC, ICC, IF, IHC, WB | |
Human, Monkey, Mouse, Rat | |
Mouse | |
Monoclonal | |
iFluor647 |
FC, ICC, IF, IHC, WB | |
Human, Monkey, Mouse, Rat | |
Mouse | |
Monoclonal | |
PE |