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Catalog Number | orb429476 |
---|---|
Category | Proteins |
Description | Recombinant of human Angiotensin II protein |
Form/Appearance | Filtered White lyophilized (freeze-dried) powder. |
Buffer/Preservatives | Filtered (0.4µm) and lyophilized from 0.5mg/ml in 20mM Tris buffer and 50mM NaCl, pH 7.5. |
Purity | Greater than 95.0% as determined by SDS-PAGE. |
Protein Sequence | DRVYIHPFHL VIHNESTCEQ LAKANAGKPK DPTFIPAPIQ AKTSPVDEKA LQDQLVLVAA KLDTEDKLRA AMVGMLANFL GFRIYGMHSE LWGVVHGATV LSPTAVFGTL ASLYLGALDH TADRLQAILG VPWKDKNCTS RLDAHKVLSA LQAVQGLLVA QGRADSQAQL LLSTVVGVFT APGLHLKQPF VQGLALYTPV VLPRSLDFTE LDVAAEKIDR FMQAVTGWKT GCSLTGASVD STLAFNTYVH FQGKMKGFSL LAEPQEFWVD NSTSVSVPML SGMGTFQHWS DIQDNFSVTQ VSFTESACLL LIQPHYASDL DKVEGLTFQQ NSLNWMKKLS PRTIHLTMPQ LVLQGSYDLQ DLLAQAELPA ILHTELNLQK LSNDRIRVGE VLNSIFFELE ADEREPTEST QQLNKPEVLE VTLNRPFLFA VYDQSATALH FLGRVANPLS TART DYKDDD DK |
Application notes | Recombinant & Natural Proteins |
Source | HEK 293 |
Solubility (25°C) | It is recommended to add deionized water to a working concentration of 0.5mg/ml and let the lyophilized pellet dissolve completely. SERPINA8 is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture. |
Storage | Stability: Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C |
Alternative names | Angiotensinogen, Serpin A8, AGT, SERPINA8, ANHU. |
Note | For research use only |
Greater than 90% as determined by SDS-PAGE. | |
57.9 kDa | |
E.coli |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
Human cells |
> 97% by SDS-PAGE. | |
Recombinant Human Angiotensinogen Protein is produced by HEK293 Cells expression system. The target protein is expressed with sequence (Asp 34 - Ala 485 ) of human Angiotensinogen (Accession #NP_000020.1) fused with a 6��His Tag at the C-terminus. |
> 98%, determined by SDS-PAGE | |
This protein contains the human angiotensinogen (NP_0000201) (Met 1-Ala 485) was fused with a polyhistidine tag at the C-terminus and expressed from HEK293 Cells. |