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Human SERPINA3 protein

SKU: orb392453

Description

Recombinant human SERPINA3 protein is produced by our Mammalian expression system and the target gene encoding His24-Ala423 is expressed with a 6His tag at the C-terminus.

Research Area

Epigenetics, Immunology

Images & Validation

Tested ApplicationsELISA, MS, SDS-PAGE, WB
Application Notes
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

SourceHuman cells
ReactivityHuman
Protein SequenceHPNSPLDEEN LTQENQDRGT HVDLGLASAN VDFAFSLYKQ LVLKAPDKNV IFSPLSISTA LAFLSLGAHN TTLTEILKGL KFNLTETSEA EIHQSFQHLL RTLNQSSDEL QLSMGNAMFV KEQLSLLDRF TEDAKRLYGS EAFATDFQDS AAAKKLINDY VKNGTRGKIT DLIKDLDSQT MMVLVNYIFF KAKWEMPFDP QDTHQSRFYL SKKKWVMVPM MSLHHLTIPY FRDEELSCTV VELRYTGNAS ALFILPDQDK MEEVEAMLLP ETLKRWRDSL EFREIGELYL PKFSISRDYN LNDILLQLGI EEAFTSKADL SGITGARNLA VSQVVHKAVL DVFEEGTEAS AATAVKITLL SALVETRTIV RFNRPFLMII VPTDTQNIFF MSKVTNPKQA VDHHHHHH
PurityGreater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
EndotoxinsLess than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Storage & Handling

StorageLyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
Form/AppearanceLyophilized from a 0.2 μM filtered solution of 20mM HEPES, 150mM NaCl, pH 7.5
DisclaimerFor research use only

Alternative Names

AACT; Alpha-1-antichymotrypsin; ACT; Cell growth-inhibiting gene 24/25 protein; Serpin A3

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UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol

Human SERPINA3 protein (orb392453)

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Available Sizes

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10 μg
$ 350.00
50 μg
$ 910.00
500 μg
$ 3,540.00
DispatchUsually dispatched within 5-10 working days
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