Human SEMA5A protein

• Catalog Number: orb392443
• Category: Proteins
• Description: Recombinant human SEMA5A protein is produced by our Mammalian expression system and the target gene encoding Glu23-Thr765 is expressed with a 6His tag at the C-terminus.
• Tested applications: ELISA, MS, SDS-PAGE, WB
• Reactivity: Human
• Form/Appearance: Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, 0.1mM EDTA, 0.05% Tween 20, pH 7.2
• Purity: Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
• Target: SEMA5A
• Entrez: 9037
• UniProt ID: Q13591
• Protein Sequence: EAQGTTQCQR TEHPVISYKE IGPWLREFRA KNAVDFSQLT FDPGQKELVV GARNYLFRLQ LEDLSLIQAV EWECDEATKK ACYSKGKSKE ECQNYIRVLL VGGDRLFTCG TNAFTPVCTN RSLSNLTEIH DQISGMARCP YSPQHNSTAL LTAGGELYAA TAMDFPGRDP AIYRSLGILP PLRTAQYNSK WLNEPNFVSS YDIGNFTYFF FRENAVEHDC GKTVFSRAAR VCKNDIGGRF LLEDTWTTFM KARLNCSRPG EVPFYYNELQ STFFLPELDL IYGIFTTNVN SIAASAVCVF NLSAIAQAFS GPFKYQENSR SAWLPYPNPN PHFQCGTVDQ GLYVNLTERN LQDAQKFILM HEVVQPVTTV PSFMEDNSRF SHVAVDVVQG REALVHIIYL ATDYGTIKKV RVPLNQTSSS CLLEEIELFP ERRREPIRSL QILHSQSVLF VGLREHVVKI PLKRCQFYRT RSTCIGAQDP YCGWDVVMKK CTSLEESLSM TQWEQSISAC PTRNLTVDGH FGVWSPWTPC THTDGSAVGS CLCRTRSCDS PAPQCGGWQC EGPGMEIANC SRNGGWTPWT SWSPCSTTCG IGFQVRQRSC SNPTPRHGGR VCVGQNREER YCNEHLLCPP HMFWTGWGPW ERCTAQCGGG IQARRRICEN GPDCAGCNVE YQSCNTNPCP ELKKTTPWTP WTPVNISDNG GHYEQRFRYT CKARLADPNL LEVGRQRIEM RYCSSDGTSG CSTLDHHHHH H
• Source: Human cells
• Endotoxins: Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
• Storage: Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
• Buffer/Preservatives: Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, 0.1mM EDTA, 0.05% Tween 20, pH 7.2
• Alternative names: SEMAF; Semaphorin-5A; Semaphorin-F; Sema F
• Note: For research use only
• Application notes: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.