Human SEMA4D protein

SKU: orb392440

Description

Recombinant human SEMA4D protein is produced by our mammalian expression system and the target gene encoding Met22-Arg734 is expressed with a Fc tag at the C-terminus.

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA, MS, SDS-PAGE, WB
• Application Notes:
  Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

• Source: Human cells
• Target: SEMA4D
• Reactivity: Human
• Endotoxins: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Protein Sequence: MAFAPIPRIT WEHREVHLVQ FHEPDIYNYS ALLLSEDKDT LYIGAREAVF AVNALNISEK QHEVYWKVSE DKKAKCAEKG KSKQTECLNY IRVLQPLSAT SLYVCGTNAF QPACDHLNLT SFKFLGKNED GKGRCPFDPA HSYTSVMVDG ELYSGTSYNF LGSEPIISRN SSHSPLRTEY AIPWLNEPSF VFADVIRKSP DSPDGEDDRV YFFFTEVSVE YEFVFRVLIP RIARVCKGDQ GGLRTLQKKW TSFLKARLIC SRPDSGLVFN VLRDVFVLRS PGLKVPVFYA LFTPQLNNVG LSAVCAYNLS TAEEVFSHGK YMQSTTVEQS HTKWVRYNGP VPKPRPGACI DSEARAANYT SSLNLPDKTL QFVKDHPLMD DSVTPIDNRP RLIKKDVNYT QIVVDRTQAL DGTVYDVMFV STDRGALHKA ISLEHAVHII EETQLFQDFE PVQTLLLSSK KGNRFVYAGS NSGVVQAPLA FCGKHGTCED CVLARDPYCA WSPPTATCVA LHQTESPSRG LIQEMSGDAS VCPDKSKGSY RQHFFKHGGT AELKCSQKSN LARVFWKFQN DVLKAESPKY GLMGRKNLLI FNLSEGDSGV YQCLSEERVK NKTVFQVVAK HVLEVKVVPK PVVAPTLSVV QTEGSRIATK VVLVASTQGS PPTPAVQATS SGAITLPPKP APTGTSCEPK IVINTVPQLH SEKTMYLKSS DNRVDDIEGR MDEPKSCDKT HTCPPCPAPE LLGGPSVFLF PPKPKDTLMI SRTPEVTCVV VDVSHEDPEV KFNWYVDGVE VHNAKTKPRE EQYNSTYRVV SVLTVLHQDW LNGKEYKCKV SNKALPAPIE KTISKAKGQP REPQVYTLPP SREEMTKNQV SLTCLVKGFY PSDIAVEWES NGQPENNYKT TPPVLDSDGS FFLYSKLTVD KSRWQQGNVF SCSVMHEALH NHYTQKSLSL SPGK

Storage & Handling

• Storage: Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μM filtered solution of PBS, pH 7.4.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

C9orf164; CD100; SEMAJ; Semaphorin-4D; A8; BB18; GR3; CD100

Quick Database Links

Gene Symbol

SEMA4D

UniProt

Q92854

NCBI Gene ID

10507