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Human SEMA4D protein
Catalog Number: orb392439
| Catalog Number | orb392439 |
|---|---|
| Category | Proteins |
| Description | Recombinant human SEMA4D protein is produced by our mammalian expression system and the target gene encoding Met22-Arg734 is expressed with a 6His tag at the C-terminus. |
| Tested applications | ELISA, MS, SDS-PAGE, WB |
| Reactivity | Human |
| Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
| Purity | Greater than 95% as determined by reducing SDS-PAGE. |
| Target | SEMA4D |
| Entrez | 10507 |
| UniProt ID | Q92854 |
| Protein Sequence | MAFAPIPRIT WEHREVHLVQ FHEPDIYNYS ALLLSEDKDT LYIGAREAVF AVNALNISEK QHEVYWKVSE DKKAKCAEKG KSKQTECLNY IRVLQPLSAT SLYVCGTNAF QPACDHLNLT SFKFLGKNED GKGRCPFDPA HSYTSVMVDG ELYSGTSYNF LGSEPIISRN SSHSPLRTEY AIPWLNEPSF VFADVIRKSP DSPDGEDDRV YFFFTEVSVE YEFVFRVLIP RIARVCKGDQ GGLRTLQKKW TSFLKARLIC SRPDSGLVFN VLRDVFVLRS PGLKVPVFYA LFTPQLNNVG LSAVCAYNLS TAEEVFSHGK YMQSTTVEQS HTKWVRYNGP VPKPRPGACI DSEARAANYT SSLNLPDKTL QFVKDHPLMD DSVTPIDNRP RLIKKDVNYT QIVVDRTQAL DGTVYDVMFV STDRGALHKA ISLEHAVHII EETQLFQDFE PVQTLLLSSK KGNRFVYAGS NSGVVQAPLA FCGKHGTCED CVLARDPYCA WSPPTATCVA LHQTESPSRG LIQEMSGDAS VCPDKSKGSY RQHFFKHGGT AELKCSQKSN LARVFWKFQN DVLKAESPKY GLMGRKNLLI FNLSEGDSGV YQCLSEERVK NKTVFQVVAK HVLEVKVVPK PVVAPTLSVV QTEGSRIATK VLVASTQGSS PPTPAVQATS SGAITLPPKP APTGTSCEPK IVINTVPQLH SEKTMYLKSS DNRVDHHHHH H |
| Source | Human cells |
| Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
| Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
| Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
| Alternative names | C9orf164; CD100; SEMAJ; Semaphorin-4D; A8; BB18; G Read more... |
| Note | For research use only |
| Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
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Human Semaphorin 4D / SEMA4D / CD100 Protein [orb257252]
Unconjugated
95%
105.4 kDa
Human SEMA4D, Fc Tag (orb257252) is expressed from human 293 cells (HEK293). It contains AA Met 22 - Arg 734 (Accession # AAH54500).
1 mg, 100 μg Human SEMA4D protein [orb392440]
ELISA, MS, SDS-PAGE, WB
Greater than 95% as determined by reducing SDS-PAGE.
Human cells
500 μg, 50 μg, 10 μgHuman SEMA4D Protein, His Tag [orb1290824]
Unconjugated
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 80 kDa after removal of the signal peptide. The apparent molecular mass of SEMA4D-His is approximately 95-130 kDa due to glycosylation.
Mammalian
100 μg, 50 μg, 10 μgRecombinant Human CD100/SEMA4D Protein, His Tag [orb1553349]
≥90% as determined by SDS-PAGE
This protein contains the human SEMA4D(Met1-Arg734) was fused with the C-terminal His Tag and expressed in Mammalian cells.
100 μg, 50 μgRecombinant Human Semaphorin 4D/SEMA4D Protein, His Tag [orb1551695]
≥90% as determined by SDS-PAGE
This protein contains the human SEMA4D(Met22-Arg734) was fused with the C-terminal His Tag and expressed in Mammalian cells.
100 μg, 50 μg
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