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Human SEMA4B protein

SKU: orb392437

Description

Recombinant human SEMA4B protein is produced by our mammalian expression system and the target gene encoding Leu39-Glu712 is expressed with a Fc, 6His tag at the C-terminus.

Research Area

Developmental Biology, Epigenetics

Images & Validation

Tested ApplicationsELISA, MS, SDS-PAGE, WB
Application Notes
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

SourceHuman cells
ReactivityHuman
Protein SequenceLSPRISLPLG SEERPFLRFE AEHISNYTAL LLSRDGRTLY VGAREALFAL SSNLSFLPGG EYQELLWGAD AEKKQQCSFK GKDPQRDCQN YIKILLPLSG SHLFTCGTAA FSPMCTYINM ENFTLARDEK GNVLLEDGKG RCPFDPNFKS TALVVDGELY TGTVSSFQGN DPAISRSQSL RPTKTESSLN WLQDPAFVAS AYIPESLGSL QGDDDKIYFF FSETGQEFEF FENTIVSRIA RICKGDEGGE RVLQQRWTSF LKAQLLCSRP DDGFPFNVLQ DVFTLSPSPQ DWRDTLFYGV FTSQWHRGTT EGSAVCVFTM KDVQRVFSGL YKEVNRETQQ WYTVTHPVPT PRPGACITNS ARERKINSSL QLPDRVLNFL KDHFLMDGQV RSRMLLLQPQ ARYQRVAVHR VPGLHHTYDV LFLGTGDGRL HKAVSVGPRV HIIEELQIFS SGQPVQNLLL DTHRGLLYAA SHSGVVQVPM ANCSLYRSCG DCLLARDPYC AWSGSSCKHV SLYQPQLATR PWIQDIEGAS AKDLCSASSV VSPSFVPTGE KPCEQVQFQP NTVNTLACPL LSNLATRLWL RNGAPVNASA SCHVLPTGDL LLVGTQQLGE FQCWSLEEGF QQLVASYCPE VVEDGVADQT DEGGSVPVII STSRVSAPAG GKASWGADRS YWKEVDDIEG RMDEPKSCDK THTCPPCPAP ELLGGPSVFL FPPKPKDTLM ISRTPEVTCV VVDVSHEDPE VKFNWYVDGV EVHNAKTKPR EEQYNSTYRV VSVLTVLHQD WLNGKEYKCK VSNKALPAPI EKTISKAKGQ PREPQVYTLP PSREEMTKNQ VSLTCLVKGF YPSDIAVEWE SNGQPENNYK TTPPVLDSDG SFFLYSKLTV DKSRWQQGNV FSCSVMHEAL HNHYTQKSLS LSPGKHHHHH H
PurityGreater than 95% as determined by reducing SDS-PAGE.
EndotoxinsLess than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Storage & Handling

StorageLyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
Form/AppearanceLyophilized from a 0.2 μM filtered solution of PBS, pH 7.4.
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

KIAA1745; SEMAC; Semaphorin-4B

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UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol

Human SEMA4B protein (orb392437)

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10 μg
$ 350.00
50 μg
$ 910.00
500 μg
$ 3,540.00
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