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Human SEMA4B protein
SKU: orb392436
Description
Research Area
Developmental Biology, Epigenetics
Images & Validation
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| Tested Applications | ELISA, MS, SDS-PAGE, WB |
|---|---|
| Application Notes |
Key Properties
−| Source | Human cells |
|---|---|
| Reactivity | Human |
| Protein Sequence | LSPRISLPLG SEERPFLRFE AEHISNYTAL LLSRDGRTLY VGAREALFAL SSNLSFLPGG EYQELLWGAD AEKKQQCSFK GKDPQRDCQN YIKILLPLSG SHLFTCGTAA FSPMCTYINM ENFTLARDEK GNVLLEDGKG RCPFDPNFKS TALVVDGELY TGTVSSFQGN DPAISRSQSL RPTKTESSLN WLQDPAFVAS AYIPESLGSL QGDDDKIYFF FSETGQEFEF FENTIVSRIA RICKGDEGGE RVLQQRWTSF LKAQLLCSRP DDGFPFNVLQ DVFTLSPSPQ DWRDTLFYGV FTSQWHRGTT EGSAVCVFTM KDVQRVFSGL YKEVNRETQQ WYTVTHPVPT PRPGACITNS ARERKINSSL QLPDRVLNFL KDHFLMDGQV RSRMLLLQPQ ARYQRVAVHR VPGLHHTYDV LFLGTGDGRL HKAVSVGPRV HIIEELQIFS SGQPVQNLLL DTHRGLLYAA SHSGVVQVPM ANCSLYRSCG DCLLARDPYC AWSGSSCKHV SLYQPQLATR PWIQDIEGAS AKDLCSASSV VSPSFVPTGE KPCEQVQFQP NTVNTLACPL LSNLATRLWL RNGAPVNASA SCHVLPTGDL LLVGTQQLGE FQCWSLEEGF QQLVASYCPE VVEDGVADQT DEGGSVPVII STSRVSAPAG GKASWGADRS YWKEVDHHHH HH |
| Purity | Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxins | Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage & Handling
−| Storage | Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid in a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
| Disclaimer | For research use only |
Alternative Names
−KIAA1745; SEMAC; Semaphorin-4B
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Unconjugated
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 75.4 kDa after removal of the signal peptide. The apparent molecular mass of SEMA4B-His is approximately 70-100 kDa due to glycosylation.
Mammalian
100 μg, 50 μg, 10 μg Recombinant Human SEMA4B Protein, N-GST [orb2967270]
>90% as determined by SDS-PAGE.
47.60 kDa
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
Human SEMA4B protein (orb392436)
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