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Catalog Number | orb392436 |
---|---|
Category | Proteins |
Description | Recombinant human SEMA4B protein is produced by our mammalian expression system and the target gene encoding Leu39-Glu712 is expressed with a 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Liquid in a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | SEMA4B |
Entrez | 10509 |
UniProt ID | Q9NPR2 |
Protein Sequence | LSPRISLPLG SEERPFLRFE AEHISNYTAL LLSRDGRTLY VGAREALFAL SSNLSFLPGG EYQELLWGAD AEKKQQCSFK GKDPQRDCQN YIKILLPLSG SHLFTCGTAA FSPMCTYINM ENFTLARDEK GNVLLEDGKG RCPFDPNFKS TALVVDGELY TGTVSSFQGN DPAISRSQSL RPTKTESSLN WLQDPAFVAS AYIPESLGSL QGDDDKIYFF FSETGQEFEF FENTIVSRIA RICKGDEGGE RVLQQRWTSF LKAQLLCSRP DDGFPFNVLQ DVFTLSPSPQ DWRDTLFYGV FTSQWHRGTT EGSAVCVFTM KDVQRVFSGL YKEVNRETQQ WYTVTHPVPT PRPGACITNS ARERKINSSL QLPDRVLNFL KDHFLMDGQV RSRMLLLQPQ ARYQRVAVHR VPGLHHTYDV LFLGTGDGRL HKAVSVGPRV HIIEELQIFS SGQPVQNLLL DTHRGLLYAA SHSGVVQVPM ANCSLYRSCG DCLLARDPYC AWSGSSCKHV SLYQPQLATR PWIQDIEGAS AKDLCSASSV VSPSFVPTGE KPCEQVQFQP NTVNTLACPL LSNLATRLWL RNGAPVNASA SCHVLPTGDL LLVGTQQLGE FQCWSLEEGF QQLVASYCPE VVEDGVADQT DEGGSVPVII STSRVSAPAG GKASWGADRS YWKEVDHHHH HH |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | Liquid in a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
Alternative names | KIAA1745; SEMAC; Semaphorin-4B Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
Human cells |
Unconjugated | |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 75.4 kDa after removal of the signal peptide. The apparent molecular mass of SEMA4B-His is approximately 70-100 kDa due to glycosylation. | |
Mammalian |
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