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Catalog Number | orb392421 |
---|---|
Category | Proteins |
Description | Recombinant human SCGN protein is produced by our mammalian expression system and the target gene encoding Met1-Pro276 is expressed with a 6His tag at the C-terminus. |
Target | SCGN |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of PBS, pH 7.4. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of PBS, pH 7.4. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Protein Sequence | MDSSREPTLG RLDAAGFWQV WQRFDADEKG YIEEKELDAF FLHMLMKLGT DDTVMKANLH KVKQQFMTTQ DASKDGRIRM KELAGMFLSE DENFLLLFRR ENPLDSSVEF MQIWRKYDAD SSGFISAAEL RNFLRDLFLH HKKAISEAKL EEYTGTMMKI FDRNKDGRLD LNDLARILAL QENFLLQFKM DACSTEERKR DFEKIFAYYD VSKTGALEGP EVDGFVKDMM ELVQPSISGV DLDKFREILL RHCDVNKDGK IQKSELALCL GLKINPVDHH HHHH |
UniProt ID | O76038 |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Source | Human cells |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Alternative names | SECRET; Secretagogin |
Note | For research use only |
Entrez | 10590 |
> 90% as determined by SDS-PAGE. | |
34.35 kDa |
ELISA, WB | |
Greater than 95% as determined by SDS-PAGE | |
30.1 kDa | |
E.Coli |
> 97%, determined by SDS-PAGE | |
This protein contains the human SCGN (NP_0089292) (Asp 2-Pro 276) was expressed with a polyhistide tag at the N-terminus and expressed from E. coli. |
> 95% by SDS-PAGE. | |
Recombinant Human SCGN Protein is produced by Mammalian expression system. The target protein is expressed with sequence (Met1-Pro276) of human SCGN (Accession #O76038) fused with a 6xHis Tag at the C- terminus. |