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Catalog Number | orb392411 |
---|---|
Category | Proteins |
Description | Recombinant human S100A8 protein is produced by our E. coli expression system and the target gene encoding Met1-Glu93 (S100A8) & Thr2-Pro114 (S100A9) is expressed with a 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Liquid in a 0.2 μM filtered solution of 20mM HEPES, 10% Glycerol, 150mM NaCl, 2.5mM EDTA, pH 7.4 |
Purity | Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
Target | S100A8; S100A9 |
Entrez | 6280, 6279 |
UniProt ID | P06702, P05109 |
Protein Sequence | MLTELEKALN SIIDVYHKYS LIKGNFHAVY RDDLKKLLET ECPQYIRKKG ADVWFKELDI NTDGAVNFQE FLILVIKMGV AAHKKSHEES HKEHHHHHH* &TCKMSQLER NIETIINTFH QYSVKLGHPD TLNQGEFKEL VRKDLQNFLK KENKNEKVIE HIMEDLDTNA DKQLSFEEFI MLMARLTWAS HEKMHEGDEG PGHHHKPGLG EGTP* |
Source | E. coli |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | Liquid in a 0.2 μM filtered solution of 20mM HEPES, 10% Glycerol, 150mM NaCl, 2.5mM EDTA, pH 7.4 |
Alternative names | CAGA CFAG MRP8 CAGB CFAG MRP14; Protein S100-A8; C Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
95% | |
10.8 kDa | |
Human S100A8, Tag Free (orb257818) is expressed from E.coli cells. It contains AA Met 1 - Glu 93 (Accession # AAH05928). |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. | |
E. coli |
Human | |
1.56 ng/mL-100 ng/mL | |
0.39 ng/mL |
Human | |
1.25 ng/mL-80 ng/mL | |
0.31 ng/mL |
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