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Human RSPO1 protein
Catalog Number: orb392401
| Catalog Number | orb392401 |
|---|---|
| Category | Proteins |
| Description | Recombinant human RSPO1 protein is produced by our mammalian expression system and the target gene encoding Arg31-Ala263 is expressed with a 6His tag at the C-terminus. |
| Target | RSPO1 |
| Reactivity | Human |
| Form/Appearance | Lyophilized from a 0.2 μM filtered solution of PBS, pH 7.4. |
| Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of PBS, pH 7.4. |
| Purity | Greater than 95% as determined by reducing SDS-PAGE. |
| Protein Sequence | RISAEGSQAC AKGCELCSEV NGCLKCSPKL FILLERNDIR QVGVCLPSCP PGYFDARNPD MNKCIKCKIE HCEACFSHNF CTKCKEGLYL HKGRCYPACP EGSSAANGTM ECSSPAQCEM SEWSPWGPCS KKQQLCGFRR GSEERTRRVL HAPVGDHAAC SDTKETRRCT VRRVPCPEGQ KRRKGGQGRR ENANRNLARK ESKEAGAGSR RRKGQQQQQQ QGTVGPLTSA GPAVDHHHHH H |
| UniProt ID | Q2MKA7 |
| Tested applications | ELISA, MS, SDS-PAGE, WB |
| Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
| Source | Human cells |
| Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
| Alternative names | R-spondin-1; Roof plate-specific spondin-1; hRspo1 |
| Note | For research use only |
| Entrez | 284654 |
- Item 1 of 2
Human RSPO1(21-146) Protein, hFc Tag [orb1290875]
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 39.9 kDa after removal of the signal peptide. The apparent molecular mass of RSPO1(21-146)-hFc is approximately 35-55 kDa due to glycosylation.
Mammalian
100 μg, 10 μg, 50 μg - Item 1 of 2
Human RSPO1 Protein [orb1477849]
Greater than 95% as determined by SDS-PAGE.
30.2 kDa
Mammalian cell
1 mg, 20 μg, 100 μg - Item 1 of 1
Human RSPO1(21-263) Protein, His Tag [orb1290876]
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 27.6 kDa after removal of the signal peptide. The apparent molecular mass of RSPO1(21-263)-His is approximately 35-55 kDa due to glycosylation.
Mammalian
100 μg, 10 μg, 50 μg - Item 1 of 1
Human RSPO1 protein [orb594926]
Greater than 95% as determined by SDS-PAGE.
27.8 kDa
Mammalian cell
1 mg, 500 μg, 50 μg, 10 μg - Item 1 of 1
Recombinant human RSPO1 protein, C-His (HEK293) [orb1516340]
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC
Due to glycosylation, the protein migrates to 38-42 kDa based on Tris-Bis PAGE result.
50 μg
