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Catalog Number | orb392394 |
---|---|
Category | Proteins |
Description | Recombinant human RNASET2 protein is produced by our mammalian expression system and the target gene encoding Asp25-His256 is expressed with a 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Liquid in a 0.2 μM filtered solution of 20mM Tris-HCl, 150mM NaCl, 20% Glycerol, pH 7.5. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | RNASET2 |
Entrez | 8635 |
UniProt ID | O00584 |
Protein Sequence | DKRLRDNHEW KKLIMVQHWP ETVCEKIQND CRDPPDYWTI HGLWPDKSEG CNRSWPFNLE EIKDLLPEMR AYWPDVIHSF PNRSRFWKHE WEKHGTCAAQ VDALNSQKKY FGRSLELYRE LDLNSVLLKL GIKPSINYYQ VADFKDALAR VYGVIPKIQC LPPSQDEEVQ TIGQIELCLT KQDQQLQNCT EPGEQPSPKQ EVWLANGAAE SRGLRVCEDG PVFYPPPKKT KHVDHHHHHH |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | Liquid in a 0.2 μM filtered solution of 20mM Tris-HCl, 150mM NaCl, 20% Glycerol, pH 7.5. |
Alternative names | RNASE6PL; Ribonuclease T2; Ribonuclease 6 Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
98.00% | |
93.5 kDa (predicted) |
98.00% | |
28.5 kDa (predicted); 35 kDa (reducing condition, due to glycosylation) |