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| Catalog Number | orb392363 |
|---|---|
| Category | Proteins |
| Description | Recombinant human PTPRN protein is produced by our E. coli expression system and the target gene encoding Met687-Gln979 is expressed with a 6His tag at the N-terminus. |
| Reactivity | Human |
| Form/Appearance | Liquid in a 0.2 μM filtered solution of 20mM Tris, 150mM NaCl, pH 8.0. |
| Purity | Greater than 95% as determined by reducing SDS-PAGE. |
| Protein Sequence | MGSSHHHHHH SSGLVPRGSH MDISTGHMIL AYMEDHLRNR DRLAKEWQAL CAYQAEPNTC ATAQGEGNIK KNRHPDFLPY DHARIKLKVE SSPSRSDYIN ASPIIEHDPR MPAYIATQGP LSHTIADFWQ MVWESGCTVI VMLTPLVEDG VKQCDRYWPD EGASLYHVYE VNLVSEHIWC EDFLVRSFYL KNVQTQETRT LTQFHFLSWP AEGTPASTRP LLDFRRKVNK CYRGRSCPII VHCSDGAGRT GTYILIDMVL NRMAKGVKEI DIAATLEHVR DQRPGLVRSK DQFEFALTAV AEEVNAILKA LPQ |
| UniProt ID | Q16849 |
| Tested applications | ELISA, MS, SDS-PAGE, WB |
| Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Endotoxins | Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
| Source | E. coli |
| Storage | Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Alternative names | ICA3; ICA512; Receptor-type tyrosine-protein phosp Read more... |
| Research Area | Epigenetics |
| Note | For research use only |
| Entrez | 5798 |
| Expiration Date | 6 months from date of receipt. |
Human | |
0.32-20 ng/mL | |
0.119 ng/mL |
Human | |
0.32-20ng/mL | |
0.19 ng/mL |
Unconjugated | |
SDS-PAGE: Greater than 90% as determined by reducing SDS-PAGE. (QC verified) | |
Predicted: 22.9 KDa. Observed: 26-30 KDa, reducing conditions |
Unconjugated | |
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. | |
Predicted: 44.6 KDa. Observed: 50 KDa, reducing conditions |
Unconjugated | |
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. | |
Predicted: 35.6 KDa. Observed: 33 KDa, reducing conditions |
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