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Catalog Number | orb392362 |
---|---|
Category | Proteins |
Description | Recombinant human PTPN6 protein is produced by our E. coli expression system and the target gene encoding Lys243-Ile541 is expressed with a 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Liquid in a 0.2 μM filtered solution of 25mM Tris-HCl, 2mM β-ME, 1mM EDTA, 1mM DTT, 20% Glycerol, pH 7.5. |
Purity | Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
Target | PTPN6 |
Entrez | 5777 |
UniProt ID | P29350 |
Protein Sequence | MKAGFWEEFE SLQKQEVKNL HQRLEGQRPE NKGKNRYKNI LPFDHSRVIL QGRDSNIPGS DYINANYIKN QLLGPDENAK TYIASQGCLE ATVNDFWQMA WQENSRVIVM TTREVEKGRN KCVPYWPEVG MQRAYGPYSV TNCGEHDTTE YKLRTLQVSP LDNGDLIREI WHYQYLSWPD HGVPSEPGGV LSFLDQINQR QESLPHAGPI IVHCSAGIGR TGTIIVIDML MENISTKGLD CDIDIQKTIQ MVRAQRSGMV QTEAQYKFIY VAIAQFIETT KKKLEVLQSQ KGQESEYGNI LEHHHHHH |
Source | E. coli |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | Liquid in a 0.2 μM filtered solution of 25mM Tris-HCl, 2mM β-ME, 1mM EDTA, 1mM DTT, 20% Glycerol, pH 7.5. |
Alternative names | HCP; PTP1C; Tyrosine-protein phosphatase non-recep Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, FC, ICC, IF, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
Greater than 90% as determined by SDS-PAGE. | |
79.1 kDa | |
E.coli |