Human PTPN6 protein

SKU: orb392362

Description

Recombinant human PTPN6 protein is produced by our E. coli expression system and the target gene encoding Lys243-Ile541 is expressed with a 6His tag at the C-terminus.

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, MS, SDS-PAGE, WB
• Application Notes:
  Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

• Source: E. coli
• Target: PTPN6
• Reactivity: Human
• Protein Sequence: MKAGFWEEFE SLQKQEVKNL HQRLEGQRPE NKGKNRYKNI LPFDHSRVIL QGRDSNIPGS DYINANYIKN QLLGPDENAK TYIASQGCLE ATVNDFWQMA WQENSRVIVM TTREVEKGRN KCVPYWPEVG MQRAYGPYSV TNCGEHDTTE YKLRTLQVSP LDNGDLIREI WHYQYLSWPD HGVPSEPGGV LSFLDQINQR QESLPHAGPI IVHCSAGIGR TGTIIVIDML MENISTKGLD CDIDIQKTIQ MVRAQRSGMV QTEAQYKFIY VAIAQFIETT KKKLEVLQSQ KGQESEYGNI LEHHHHHH
• Purity: Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
• Endotoxins: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Storage & Handling

• Storage: Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.2 μM filtered solution of 25mM Tris-HCl, 2mM β-ME, 1mM EDTA, 1mM DTT, 20% Glycerol, pH 7.5.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HCP; PTP1C; Tyrosine-protein phosphatase non-receptor type 6; Hematopoietic cell protein-tyrosine phosphatase; Protein-tyrosine phosphatase 1C; PTP-1C; Protein-tyrosine phosphatase SHP-1; SH-PTP1

Quick Database Links

Gene Symbol

PTPN6

UniProt

P29350

NCBI Gene ID

5777