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Catalog Number | orb427963 |
---|---|
Category | Proteins |
Description | Recombinant of human PTH (1-84) protein |
Form/Appearance | Lyophilized from a 0.2µm filtered concentrated solution in PBS pH7.4. |
Purity | Greater than 95.0% as determined by, analysis by RP-HPLC and analysis by SDS-PAGE |
MW | 9.4kDa |
Protein Sequence | SVSEIQLMHN LGKHLNSMER VEWLRKKLQD VHNFVALGAP LAPRDAGSQR PRKKEDNVLV ESHEKSLGEA DKADVNVLTK AKSQ |
Source | E.coli |
Biological Activity | The activity is calculated by its ability to induce cAMP accumulation in murine MC3T3E1 cells and is < 50ng/ml, corresponding to a specific activity of greater than 2.0 x 104 Units/mg |
Storage | Lyophilized Parathyrin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PTH should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles |
Buffer/Preservatives | It is recommended to reconstitute the lyophilized Parathormone in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions |
Note | For research use only |
Application notes | Hormones |
Expiration Date | 6 months from date of receipt. |
FA, HPLC, SDS-PAGE | |
Unconjugated | |
> 97.0% as determined by RP-HPLC and analysis by SDS-PAGE |
HPLC, SDS-PAGE | |
Unconjugated | |
> 97 % by SDS-PAGE and HPLC analyses | |
9.5 kDa |
> 95% by SDS-PAGE. | |
KMP1621, Recombinant Human PTH 1-84/Parathyroid hormone/Parathormone Protein is produced by E. coli expression system. The target protein is expressed with sequence (Ser32-Gln115) of human PTH 1-84/Parathyroid hormone/Parathormone (Accession #P01270). |
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