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Catalog Number | orb427833 |
---|---|
Category | Tools |
Description | Recombinant of human PRDX4 protein |
Form/Appearance | Sterile filtered colorless solution. |
Purity | Greater than 95% as determined by SDS-PAGE. |
Protein Sequence | MGSSHHHHHH SSGLVPRGSH MWETEERPRT REEECHFYAG GQVYPGEASR VSVADHSLHL SKAKISKPAP YWEGTAVIDG EFKELKLTDY RGKYLVFFFY PLDFTFVCPT EIIAFGDRLE EFRSINTEVV ACSVDSQFTH LAWINTPRRQ GGLGPIRIPL LSDLTHQISK DYGVYLEDSG HTLRGLFIID DKGILRQITL NDLPVGRSVD ETLRLVQAFQ YTDKHGEVCP AGWKPGSETI IPDPAGKLKY FDKLN |
Source | Escherichia Coli |
Biological Activity | Specific activity: approximately 230-310 pmole/min/µg.Enzymatic activity was confirmed by measuring the remaining peroxide after incubation of PRDX4 and peroxide for 20 min at room temperature. Specific activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25 C for 1 minute. |
Storage | Stability: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles |
Buffer/Preservatives | PRDX4 Human solution containing 20mM Tris-HCl pH-8 & 10% glycerol. |
Alternative names | EC 1.11.1.15, AOE37-2, Peroxiredoxin-IV, Prx-IV, T Read more... |
Note | For research use only |
Application notes | Enzymes |
Expiration Date | 12 months from date of receipt. |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
E. coli |
ELISA, WB | |
Greater than 95% as determined by SDS-PAGE | |
26.6 kDa | |
E.Coli |
≥90% as determined by SDS-PAGE | |
This protein contains the human PRDX4(Met1-Asn271) was fused with the C-terminal His Tag N-terminal His Tag and expressed in E. coli. |
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