Human PPT1 protein

SKU: orb392324

Description

Recombinant human PPT1 protein is produced by our mammalian expression system and the target gene encoding Asp28-Gly306 is expressed with a 6His tag at the C-terminus.

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, MS, SDS-PAGE, WB
• Application Notes:
  Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

• Source: Human cells
• Target: PPT1
• Reactivity: Human
• Endotoxins: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Protein Sequence: DPPAPLPLVI WHGMGDSCCN PLSMGAIKKM VEKKIPGIYV LSLEIGKTLM EDVENSFFLN VNSQVTTVCQ ALAKDPKLQQ GYNAMGFSQG GQFLRAVAQR CPSPPMINLI SVGGQHQGVF GLPRCPGESS HICDFIRKTL NAGAYSKVVQ ERLVQAEYWH DPIKEDVYRN HSIFLADINQ ERGINESYKK NLMALKKFVM VKFLNDSIVD PVDSEWFGFY RSGQAKETIP LQETSLYTQD RLGLKEMDNA GQLVFLATEG DHLQLSEEWF YAHIIPFLGV DHHHHHH

Storage & Handling

• Storage: Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.2 μM filtered solution of 20mM Tris-HCl, 150mM NaCl, 10% Glycerol, pH 7.5.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PPT; Palmitoyl-protein thioesterase 1; PPT-1; Palmitoyl-protein hydrolase 1

Quick Database Links

Gene Symbol

PPT1

UniProt

P50897

NCBI Gene ID

5538