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Description
Research Area
Cell Biology
Images & Validation
−
| Tested Applications | ELISA, MS, SDS-PAGE, WB |
|---|---|
| Application Notes |
Key Properties
−| Source | Human cells |
|---|---|
| Target | PPT1 |
| Reactivity | Human |
| Endotoxins | Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
| Purity | Greater than 95% as determined by reducing SDS-PAGE. |
| Protein Sequence | DPPAPLPLVI WHGMGDSCCN PLSMGAIKKM VEKKIPGIYV LSLEIGKTLM EDVENSFFLN VNSQVTTVCQ ALAKDPKLQQ GYNAMGFSQG GQFLRAVAQR CPSPPMINLI SVGGQHQGVF GLPRCPGESS HICDFIRKTL NAGAYSKVVQ ERLVQAEYWH DPIKEDVYRN HSIFLADINQ ERGINESYKK NLMALKKFVM VKFLNDSIVD PVDSEWFGFY RSGQAKETIP LQETSLYTQD RLGLKEMDNA GQLVFLATEG DHLQLSEEWF YAHIIPFLGV DHHHHHH |
Storage & Handling
−| Storage | Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.2 μM filtered solution of 20mM Tris-HCl, 150mM NaCl, 10% Glycerol, pH 7.5. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−PPT; Palmitoyl-protein thioesterase 1; PPT-1; Palmitoyl-protein hydrolase 1
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Human PPT1 Protein, His Tag [orb1290827]
Unconjugated
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass 32.1 of kDa after removal of the signal peptide. The apparent molecular mass of PPT1-His is approximately 33-40 kDa due to glycosylation.
Mammalian
10 μg, 50 μg, 100 μg
Quality Guarantee
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
Available Sizes
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