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| Catalog Number | orb427806 |
|---|---|
| Category | Proteins |
| Description | Recombinant of human PPM1A protein |
| Form/Appearance | Sterile filtered colorless solution. |
| Buffer/Preservatives | PPM1A protein solution (1mg/ml) contains 10mM Tris-HCl, pH 7.5, 50mM NaCl, 2mM DTT, 1mM MnCl2 and 20% glycerol. |
| Purity | Greater than 95.0% as determined by SDS-PAGE. |
| Protein Sequence | MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWILMGAF LDKPKMEKHN AQGQGNGLRY GLSSMQGWRV EMEDAHTAVI GLPSGLESWS FFAVYDGHAG SQVAKYCCEH LLDHITNNQD FKGSAGAPSV ENVKNGIRTG FLEIDEHMRV MSEKKHGADR SGSTAVGVLI SPQHTYFINC GDSRGLLCRN RKVHFFTQDH KPSNPLEKER IQNAGGSVMI QRVNGSLAVS RALGDFDYKC VHGKGPTEQL VSPEPEVHDI ERSEEDDQFI ILACDGIWDV MGNEELCDFV RSRLEVTDDL EKVCNEVVDT CLYKGSRDNM SVILICFPNA PKVSPEAVKK EAELDKYLEC RVEEIIKKQG EGVPDLVHVM RTLASENIPS LPPGGELASK RNVIEAVYNR LNPYKNDDTD STSTDDMW |
| Application notes | Unit Definition: One unit will hydrolyze 1 nanomole of p-nitrophenyl phosphatate (pNPP) per minute at pH 7.5 at 37°C |
| Source | Escherichia Coli |
| Storage | Stability: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles |
| Alternative names | Protein phosphatase 1A, EC 3.1.3.16, Protein phosp Read more... |
| Note | For research use only |
Human | |
0.32-20 ng/mL | |
0.126 ng/mL |
Unconjugated | |
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. | |
Predicted: 43.5 KDa. Observed: 45 KDa, reducing conditions |
Greater than 90% as determined by SDS-PAGE. | |
69.3 kDa | |
E.coli |
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