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Catalog Number | orb427764 |
---|---|
Category | Proteins |
Description | Recombinant of human POLB protein |
Form/Appearance | Sterile filtered colorless solution. |
Buffer/Preservatives | POLB protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 30% glycerol and 0.1M NaCl. |
Purity | Greater than 95.0% as determined by SDS-PAGE. |
Protein Sequence | MGSSHHHHHH SSGLVPRGSH MSKRKAPQET LNGGITDMLT ELANFEKNVS QAIHKYNAYR KAASVIAKYP HKIKSGAEAK KLPGVGTKIA EKIDEFLATG KLRKLEKIRQ DDTSSSINFL TRVSGIGPSA ARKFVDEGIK TLEDLRKNED KLNHHQRIGL KYFGDFEKRI PREEMLQMQD IVLNEVKKVD SEYIATVCGS FRRGAESSGD MDVLLTHPSF TSESTKQPKL LHQVVEQLQK VHFITDTLSK GETKFMGVCQ LPSKNDEKEY PHRRIDIRLI PKDQYYCGVL YFTGSDIFNK NMRAHALEKG FTINEYTIRP LGVTGVAGEP LPVDSEKDIF DYIQWKYREP KDRSE |
Application notes | Enzymes |
Source | Escherichia Coli |
Storage | Stability: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles |
Alternative names | DNA polymerase beta, DNA directed DNA polymerase b Read more... |
Note | For research use only |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. | |
E. coli |
> 90% as determined by SDS-PAGE. | |
39.2 kDa |
ELISA, WB | |
Greater than 95% as determined by SDS-PAGE | |
36.7 kDa | |
E.Coli |
≥90% as determined by SDS-PAGE | |
This protein contains the human POLB(Ser2-Glu335) was fused with the C-terminal His Tag and expressed in E. coli. |