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Human POLB protein
Catalog Number: orb392306
| Catalog Number | orb392306 |
|---|---|
| Category | Proteins |
| Description | Recombinant human POLB protein is produced by our E. coli expression system and the target gene encoding Ser2-Glu335 is expressed with a 6His tag at the C-terminus. |
| Target | POLB |
| Reactivity | Human |
| Form/Appearance | Liquid in a 0.2 μM filtered solution of 20mM Tris-HCl, 100mM NaCl, 1mM DTT, 1mM EDTA, 50% Glycerol, pH 7.8. |
| Buffer/Preservatives | Liquid in a 0.2 μM filtered solution of 20mM Tris-HCl, 100mM NaCl, 1mM DTT, 1mM EDTA, 50% Glycerol, pH 7.8. |
| Purity | Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
| Protein Sequence | MSKRKAPQET LNGGITDMLT ELANFEKNVS QAIHKYNAYR KAASVIAKYP HKIKSGAEAK KLPGVGTKIA EKIDEFLATG KLRKLEKIRQ DDTSSSINFL TRVSGIGPSA ARKFVDEGIK TLEDLRKNED KLNHHQRIGL KYFGDFEKRI PREEMLQMQD IVLNEVKKVD SEYIATVCGS FRRGAESSGD MDVLLTHPSF TSESTKQPKL LHQVVEQLQK VHFITDTLSK GETKFMGVCQ LPSKNDEKEY PHRRIDIRLI PKDQYYCGVL YFTGSDIFNK NMRAHALEKG FTINEYTIRP LGVTGVAGEP LPVDSEKDIF DYIQWKYREP KDRSEVEHHH HHH |
| UniProt ID | P06746 |
| Tested applications | ELISA, MS, SDS-PAGE, WB |
| Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
| Source | E. coli |
| Storage | Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Alternative names | DNA polymerase beta |
| Note | For research use only |
| Entrez | 5423 |
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Recombinant Human POLB Protein, N-His [orb2833476]
> 90% as determined by SDS-PAGE.
39.2 kDa
100 μg, 50 μg, 20 μgHuman DNA Polymerase beta protein [orb753924]
ELISA, WB
Greater than 95% as determined by SDS-PAGE
36.7 kDa
E.Coli
1 mg, 100 μg, 200 μg, 50 μgRecombinant Human DNA Polymeraseβ/POLB Protein, His Tag [orb1552534]
≥90% as determined by SDS-PAGE
This protein contains the human POLB(Ser2-Glu335) was fused with the C-terminal His Tag and expressed in E. coli.
50 μg, 100 μgHuman POLB Protein [orb427764]
Greater than 95.0% as determined by SDS-PAGE.
Escherichia Coli
1 mg, 20 μg, 5 μg
