Human PMVK protein

SKU: orb392303

Description

Recombinant human PMVK protein is produced by our E.coli expression system and the target gene encoding Met1-Leu192 is expressed with a 6His tag at the N-terminus.

Images & Validation

• Tested Applications: ELISA, MS, SDS-PAGE, WB
• Application Notes:
  Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

• Source: E. coli
• Reactivity: Human
• Protein Sequence: MGSSHHHHHH SSGLVPRGSH MAPLGGAPRL VLLFSGKRKS GKDFVTEALQ SRLGADVCAV LRLSGPLKEQ YAQEHGLNFQ RLLDTSTYKE AFRKDMIRWG EEKRQADPGF FCRKIVEGIS QPIWLVSDTR RVSDIQWFRE AYGAVTQTVR VVALEQSRQQ RGWVFTPGVD DAESECGLDN FGDFDWVIEN HGVEQRLEEQ LENLIEFIRS RL
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxins: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Storage & Handling

• Storage: Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Form/Appearance: Liquid in a 0.2 μM filtered solution of 20mM Tris-HCl, 100mM NaCl, 1mM DTT, 10% Glycerol, pH 7.5.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PMKI; Phosphomevalonate kinase; PMKase; hPMK

Quick Database Links

UniProt

Q15126

NCBI Gene ID

10654