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| Catalog Number | orb427741 |
|---|---|
| Category | Proteins |
| Description | Recombinant of human PLGF1 (Sf9) protein |
| Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Buffer/Preservatives | Lyophilized from a concentrated (1mg/ml) solution containing 50mM acetic acid. |
| Purity | Greater than 95.0% as determined by SDS-PAGE. |
| Protein Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Pro-Ala-Val |
| Application notes | Cytokines And Growth Factors |
| Source | Insect Cells |
| Biological Activity | The ED50 as determined by its ability to bind to immobilized rh-sFlt-1 in a functional ELISA. PlGF-1 human Recombinant can bind to immobilized rh-sFlt-1 (100ng/well) with a linear range at 0.5-10ng/ml, corresponding to a Specific Activity of 1x105-2x106units/mg. |
| Solubility (25°C) | It is recommended to reconstitute the lyophilized Placenta Growth Factor 1 in sterile 0.1M acetic acid not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Storage | Stability: Lyophilized Placenta Growth Factor 1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PLGF1 should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles |
| Alternative names | PIGF, PGF, PLGF-1. |
| Note | For research use only |
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