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Catalog Number | orb392295 |
---|---|
Category | Proteins |
Description | Recombinant human PLAU protein is produced by our Mammalian expression system and the target gene encoding Ser21-Leu431 is expressed with a 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Liquid in a 0.2 μM filtered solution of 20mM HEPES, 2mM CaCl2, 10% Glycerol, pH 7.4. |
Purity | Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
Target | PLAU |
Entrez | 5328 |
UniProt ID | P00749 |
Protein Sequence | SNELHQVPSN CDCLNGGTCV SNKYFSNIHW CNCPKKFGGQ HCEIDKSKTC YEGNGHFYRG KASTDTMGRP CLPWNSATVL QQTYHAHRSD ALQLGLGKHN YCRNPDNRRR PWCYVQVGLK PLVQECMVHD CADGKKPSSP PEELKFQCGQ KTLRPRFKII GGEFTTIENQ PWFAAIYRRH RGGSVTYVCG GSLISPCWVI SATHCFIDYP KKEDYIVYLG RSRLNSNTQG EMKFEVENLI LHKDYSADTL AHHNDIALLK IRSKEGRCAQ PSRTIQTICL PSMYNDPQFG TSCEITGFGK ENSTDYLYPE QLKMTVVKLI SHRECQQPHY YGSEVTTKML CAADPQWKTD SCQGDSGGPL VCSLQGRMTL TGIVSWGRGC ALKDKPGVYT RVSHFLPWIR SHTKEENGLA LVDHHHHHH |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | Liquid in a 0.2 μM filtered solution of 20mM HEPES, 2mM CaCl2, 10% Glycerol, pH 7.4. |
Alternative names | Urokinase-type plasminogen activator; U-plasminoge Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
92% | |
47.2 kDa | |
Human PLAU, His Tag (orb257779) is expressed from human 293 cells (HEK293). It contains AA Ser 21 - Leu 431 (Accession # NP_002649.1). |
Greater than 85% as determined by SDS-PAGE. | |
52.4 kDa | |
E.coli |
Greater than 90% as determined by SDS-PAGE. | |
21.3 kDa | |
E.coli |
Unconjugated | |
90% | |
15.3 kDa, 17.8 kDa and 29.2 kDa | |
Human PLAU, His Tag (activated by trypsin) (orb613660) is expressed from human 293 cells (HEK293). It contains AA Ser 21 - Leu 431 (Accession # P00749-1). |
> 97% by SDS-PAGE. | |
KMP1149, Recombinant Human Urokinase/PLAU Protein is produced by HEK293 Cells expression system. The target protein is expressed with sequence (Met1-Leu431) of human Urokinase/PLAU (Accession #NP_002649.1) fused with a 8×His Tag at the C-terminus. |
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