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Catalog Number | orb392294 |
---|---|
Category | Proteins |
Description | Recombinant human PLAT protein is produced by our mammalian expression system and the target gene encoding Ser36-Pro562 is expressed with a 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM MES, 150mM NaCl, 0.2mM GaCl2, pH 5.5. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | PLAT |
Entrez | 5327 |
UniProt ID | P00750 |
Protein Sequence | SYQVICRDEK TQMIYQQHQS WLRPVLRSNR VEYCWCNSGR AQCHSVPVKS CSEPRCFNGG TCQQALYFSD FVCQCPEGFA GKCCEIDTRA TCYEDQGISY RGTWSTAESG AECTNWNSSA LAQKPYSGRR PDAIRLGLGN HNYCRNPDRD SKPWCYVFKA GKYSSEFCST PACSEGNSDC YFGNGSAYRG THSLTESGAS CLPWNSMILI GKVYTAQNPS AQALGLGKHN YCRNPDGDAK PWCHVLKNRR LTWEYCDVPS CSTCGLRQYS QPQFRIKGGL FADIASHPWQ AAIFAKHRRS PGERFLCGGI LISSCWILSA AHCFQERFPP HHLTVILGRT YRVVPGEEEQ KFEVEKYIVH KEFDDDTYDN DIALLQLKSD SSRCAQESSV VRTVCLPPAD LQLPDWTECE LSGYGKHEAL SPFYSERLKE AHVRLYPSSR CTSQHLLNRT VTDNMLCAGD TRSGGPQANL HDACQGDSGG PLVCLNDGRM TLVGIISWGL GCGQKDVPGV YTKVTNYLDW IRDNMRPVDH HHHHH |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM MES, 150mM NaCl, 0.2mM GaCl2, pH 5.5. |
Alternative names | Tissue-type plasminogen activator; t-PA; t-plasmin Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Greater than 85% as determined by SDS-PAGE. | |
62.9 kDa | |
Baculovirus |
Greater than 85% as determined by SDS-PAGE. | |
62.9 kDa | |
Baculovirus |
Greater than 90% as determined by SDS-PAGE. | |
43.1 kDa | |
E.coli |
Greater than 90% as determined by SDS-PAGE. | |
74.3 kDa | |
E.coli |
Unconjugated | |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 59.9 kDa after removal of the signal peptide. The apparent molecular mass of PLAT-His is approximately 55-70 kDa due to glycosylation. | |
Mammalian |
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