Human PH20 / SPAM1 Protein

• Catalog Number: orb1146978
• Category: Proteins
• Description: Hyaluronidase PH-20 is also known as Sperm adhesion molecule 1 (SPAM1) and Sperm surface protein PH-20, which belongs to the glycosyl hydrolase 56 family, SPAM1 / PH-20 is expressed in testis. SPAM-1 / PH20 random hydrolysis of (1->4)-linkages between N – acetyl – beta – D – glucosamine and D-glucuronate residues in hyaluronate. SPAM-1 / PH20 involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. SPAM1 aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.
• Reactivity: Human
• Tag: This protein carries no "tag"
• Form/Appearance: Powder
• Purity: The purity of the protein is greater than 95%
• Conjugation: Unconjugated
• MW: Calculated MW of 52.6 kDa
• Target: PH20
• Protein Sequence: NP_694859.1
• Source: Human PH20, Tag Free (orb1146978) is expressed from human 293 cells (HEK293). It contains AA Leu 36 - Ser 490 (Accession # P38567-1).
• Expression System: HEK293
• Biological Activity: The activity of PH20 is measured by its ability to hydrolyze HA in turbidimetric assay (45 minute assay). The specific activity is > 55000 U/mg. (Unit Definition: One unit of Hyaluronidase activity will cause a change in A600 of 0.330 per minute at pH5.35 at 37 °C in a 2.0 mL reaction mixture)
• Expression Region: Predicted N-terminus:The
• Endotoxins: Less than 1.0 EU per μg by the LAL method
• NCBI: NP_694859.1
• Storage: -20°C to -70°C for 12 months in lyophilized state, -70°C for 3 months under sterile conditions after reconstitution
• Buffer/Preservatives: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM NaCl, pH7.5 with trehalose as protectant
• Alternative names: SPAM1,PH-20,HYAL3,HYA1,HYAL1,HYAL5,SPAG15
• Note: For research use only
• Application notes: This protein carries no "tag". The protein has a calculated MW of 52.6 kDa. The protein migrates as 65-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

Human PH20, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS - The purity of Human PH20, Tag Free is more than 95% and the molecular weight of this protein is around 53-71 kDa verified by SEC-MALS.