Human PH20 / SPAM1 Protein DMF Filed

• Catalog Number: orb257775
• Category: Proteins
• Description: Hyaluronidase PH-20 is also known as Sperm adhesion molecule 1 (SPAM1) and Sperm surface protein PH-20, which belongs to the glycosyl hydrolase 56 family, SPAM1 / PH-20 is expressed in testis. SPAM-1 / PH20 random hydrolysis of (1->4)-linkages between N – acetyl – beta – D – glucosamine and D-glucuronate residues in hyaluronate. SPAM-1 / PH20 involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. SPAM1 aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.
• Reactivity: Human
• Tag: C-10×His
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 53.0 kDa
• Target: PH20
• Protein Sequence: NP_694859.1
• Source: Human PH20, His Tag (orb257775) is expressed from human 293 cells (HEK293). It contains AA Leu 36 - Tyr 482 (Accession # P38567-1).
• Expression System: HEK293
• Expression Region: Leu 36 - Tyr 482
• Endotoxins: 1.0 EU per μg
• NCBI: NP_694859.1
• Storage: -20℃
• Buffer/Preservatives: 50 mM Tris, 100 mM NaCl, pH7.5
• Alternative names: SPAM1,PH-20,HYAL3,HYA1,HYAL1,HYAL5,SPAG15
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 53.0 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human PH20 protein