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Catalog Number | orb392277 |
---|---|
Category | Proteins |
Description | Recombinant human PGK1 protein is produced by our mammalian expression system and the target gene encoding Ser2-Ile417 is expressed with a 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Liquid in a 0.2 μM filtered solution of 20mM Tris, 150mM NaCl, 20% glycerol, pH 8.0. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | PGK1 |
Entrez | 5230 |
UniProt ID | P00558 |
Protein Sequence | SLSNKLTLDK LDVKGKRVVM RVDFNVPMKN NQITNNQRIK AAVPSIKFCL DNGAKSVVLM SHLGRPDGVP MPDKYSLEPV AVELKSLLGK DVLFLKDCVG PEVEKACANP AAGSVILLEN LRFHVEEEGK GKDASGNKVK AEPAKIEAFR ASLSKLGDVY VNDAFGTAHR AHSSMVGVNL PQKAGGFLMK KELNYFAKAL ESPERPFLAI LGGAKVADKI QLINNMLDKV NEMIIGGGMA FTFLKVLNNM EIGTSLFDEE GAKIVKDLMS KAEKNGVKIT LPVDFVTADK FDENAKTGQA TVASGIPAGW MGLDCGPESS KKYAEAVTRA KQIVWNGPVG VFEWEAFARG TKALMDEVVK ATSRGCITII GGGDTATCCA KWNTEDKVSH VSTGGGASLE LLEGKVLPGV DALSNILDHH HHHH |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | Liquid in a 0.2 μM filtered solution of 20mM Tris, 150mM NaCl, 20% glycerol, pH 8.0. |
Alternative names | PGKA; Phosphoglycerate kinase 1; Cell migration-in Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Greater than 90% as determined by SDS-PAGE. | |
68.9 kDa | |
E.coli |
Human | |
28 pg/mL-1800 pg/mL | |
7 pg/mL |
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